@article{5a03c1f7bbff49f48854cb9678f45352,
title = "Capturing nuclear sequence-specific DNA-binding proteins by using simian virus 40-derived minichromosomes",
abstract = "We have used recombinant simian virus 40 (SV40) minichromosomes to retrieve sequence-specific DNA-binding proteins derived from the cell nucleus of COS-7 cells. We showed that the transcription factors AP-1 and Sp1 are stably bound to the SV40 DNA late in viral infection. Under similar conditions, minichromosomes carrying the rat insulin (rINS1) enhancer, which is under negative regulation in COS-7 cells, bound two proteins which mapped to distinct regions of the rINS1 enhancer. The SV40 P element competed for one of these proteins which bound to the region from -198 to -230. This factor may be related to AP-1. The other factor selectively bound a regulatory element in the region from -92 to -124 of the insulin enhancer. These proteins may play a role in regulating the rINS1 enhancer function.",
author = "U. Nir and E. Fodor and Rutter, {W. J.}",
year = "1988",
month = feb,
doi = "10.1128/MCB.8.2.982",
language = "אנגלית",
volume = "8",
pages = "982--987",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "Taylor and Francis Ltd.",
number = "2",
}