Ca 2+-induced PARP-1 activation and ANF expression are coupled events in cardiomyocytes

Ilona Geistrikh, Leonid Visochek, Rodika Klein, Liron Miller, Leonid Mittelman, Asher Shainberg, Malka Cohen-Armon

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


The nuclear protein PARP-1 [poly(ADP-ribose) polymerase-1] is activated in cardiomyocytes exposed to hypoxia causing DNA breaks. Unlike this stress-induced PARP-1 activation, our results provide evidence for Ca 2+-induced PARP-1 activation in contracting newborn cardiomyocytes treated with growth factors and hormones that increased their contraction rate, induced intracellular Ca 2+ mobilization and its rhythmical and transient translocation into the nucleus. Furthermore, activated PARP-1 up-regulated the activity of phosphorylated ERK (extracellular-signal-regulated kinase) in the nucleus, promoting expression of the Elk1 target gene c-fos. Up-regulation of the transcription factor c-Fos/GATA-4 promoted ANF (atrial natriuretic factor) expression. Given that expression of ANF is known to be implicated in morphological changes, growth and development of cardiomyocytes, these results outline a PARP-1-dependent signal transduction mechanism that links contraction rate and Ca 2+ mobilization with the expression of genes underlying morphological changes in cardiomyocytes.

Original languageEnglish
Pages (from-to)337-347
Number of pages11
JournalBiochemical Journal
Issue number2
StatePublished - 1 Sep 2011


  • Atrial natriuretic factor (ANF) expression
  • Calcium translocation
  • Calcium-induced poly(ADP-ribose) polymerase (PARP)-1 activation
  • Newborn cardiomyocyte
  • c-fos expression


Dive into the research topics of 'Ca 2+-induced PARP-1 activation and ANF expression are coupled events in cardiomyocytes'. Together they form a unique fingerprint.

Cite this