Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Kaushalya Ghosh; Rajesh Kumar; Jarnail Singh; S. K. Gahlawat; Dharmendra Kumar; Naresh Lalaji Selokar; S. P. Yadav; B. R. Gulati; P. S. Yadav

doi:10.1007/s11626-015-9920-0

Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Kaushalya Ghosh, Rajesh Kumar, Jarnail Singh, S. K. Gahlawat, Dharmendra Kumar, Naresh Lalaji Selokar, S. P. Yadav, B. R. Gulati, P. S. Yadav

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.

Original language	English
Pages (from-to)	915-921
Number of pages	7
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	51
Issue number	9
DOIs	https://doi.org/10.1007/s11626-015-9920-0
State	Published - 1 Oct 2015
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2015, The Society for In Vitro Biology.

Funding

The present work was funded by institutional research project grant, ICAR-Central Institute for Research on Buffaloes to Dr. PS Yadav. KG is a recipient of University Research Scholarship from Department of Biotechnology, Chaudhary Devi Lal University, Sirsa-India.

Funders	Funder number
ICAR-Central

Keywords

Amnion
Buffalo
Differentiation
Stem cell

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10.1007/s11626-015-9920-0

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title = "Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro",

abstract = "Recent studies suggested that placentae amniotic membrane is a valuable source of stem cells in human as well as in livestock species. Advantages of amnion over other sources of stem cells included abundant availability, ethically non-objectionable and non-invasive source. The aim of the present study was the isolation, culture and characterization of amniotic-membrane-derived mesenchymal stem cells from term placentae collected postpartum in buffalo. We have observed that both presumptive epithelial-like and fibroblast-like cells were cultured and maintained from term amnion. These cells were shown the positive expression of pluripotency markers (OCT-4, SOX-2, NANOG, TERT), mesenchymal stem cell markers (CD29, CD44, CD105) and negative for haematopoietic marker (CD34) genes at different passages. In addition, these cells were also positive for alkaline phosphatase staining. Stem-ness potential of any stem cells is determined by their potential to differentiate into specific lineages of cell type. In the present study, we have successfully differentiated the amniotic-membrane-derived cells into adipogenic, chondrogenic and osteogenic lineages of cells in vitro. In conclusion, the results of this study demonstrate that amniotic-membrane-derived cells expressed pluripotent and mesenchymal stem cells markers and have propensity to differentiate into cells of mesenchymal lineage cell type upon directed differentiation in vitro.",

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author = "Kaushalya Ghosh and Rajesh Kumar and Jarnail Singh and Gahlawat, {S. K.} and Dharmendra Kumar and Selokar, {Naresh Lalaji} and Yadav, {S. P.} and Gulati, {B. R.} and Yadav, {P. S.}",

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AU - Ghosh, Kaushalya

AU - Kumar, Rajesh

AU - Singh, Jarnail

AU - Gahlawat, S. K.

AU - Kumar, Dharmendra

AU - Selokar, Naresh Lalaji

AU - Yadav, S. P.

AU - Gulati, B. R.

AU - Yadav, P. S.

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Buffalo (Bubalus bubalis) term amniotic-membrane-derived cells exhibited mesenchymal stem cells characteristics in vitro

Abstract

Bibliographical note

Funding

Keywords

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