TY - GEN
T1 - Broadband visible light induced NO formation
AU - Lubart, Rachel
AU - Eichler, Maor
AU - Friedmann, Harry
AU - Savion, N.
AU - Breitbart, Haim
AU - Ankri, Rinat
PY - 2009
Y1 - 2009
N2 - Nitric oxide formation is a potential mechanism for photobiomodulation because it is synthesized in cells by nitric oxide synthase (NOS), which contains both flavin and heme, and thus absorbs visible light. The purpose of this work was to study broadband visible light induced NO formation in various cells. Cardiac, endothelial, sperm cells and RAW 264.7 macrophages were illuminated with broadband visible light, 40-130mW/cm2, 2.4-39J/cm2, and nitric oxide production was quantified by using the Griess reagent. The results showed that visible light illumination increased NO concentration both in sperm and endothelial cells, but not in cardiac cells. Activation of RAW 264.7 macrophages was very small. It thus appears that NO is involved in photobiomodulation, though different light parameters and illumination protocols are needed to induce NO in various cells.
AB - Nitric oxide formation is a potential mechanism for photobiomodulation because it is synthesized in cells by nitric oxide synthase (NOS), which contains both flavin and heme, and thus absorbs visible light. The purpose of this work was to study broadband visible light induced NO formation in various cells. Cardiac, endothelial, sperm cells and RAW 264.7 macrophages were illuminated with broadband visible light, 40-130mW/cm2, 2.4-39J/cm2, and nitric oxide production was quantified by using the Griess reagent. The results showed that visible light illumination increased NO concentration both in sperm and endothelial cells, but not in cardiac cells. Activation of RAW 264.7 macrophages was very small. It thus appears that NO is involved in photobiomodulation, though different light parameters and illumination protocols are needed to induce NO in various cells.
UR - http://www.scopus.com/inward/record.url?scp=70450197326&partnerID=8YFLogxK
U2 - 10.1063/1.3175628
DO - 10.1063/1.3175628
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AN - SCOPUS:70450197326
SN - 9780735406797
T3 - AIP Conference Proceedings
SP - 49
EP - 52
BT - Laser Florence 2008 - A Bridge to the Laser Medicine World
T2 - International Laser Medicine Congress, Laser Florence 2008: A Bridge To The Laser Medicine World
Y2 - 31 October 2008 through 1 November 2008
ER -