TY - JOUR
T1 - Blocking of Pseudomonas aeruginosa lectins by human milk glycans
AU - Lesman-Movshovich, Efrat
AU - Lerrer, Batia
AU - Gilboa-Garber, Nechama
PY - 2003/3/1
Y1 - 2003/3/1
N2 - The opportunistic human pathogen Pseudomonas aeruginosa produces a D-galactophilic (PA-IL) lectin and another lectin (PA-IIL) that binds L-fucose > D-arabinose > D-mannose in close association with its host-attacking factors. These lectins contribute to the virulence of P. aeruginosa by their involvement in the production, adhesion, and pathogenic effects of its biofilm on host cells. Therefore, they are considered targets for anti-Pseudomonas therapy. The present study compares their blocking by human milk samples with that of the plant lectin Con A. It demonstrates that human milk inhibits the hemagglutinating activities of the three lectins, with PA-IIL much more strongly inhibited than PA-IL or Con A. Using these lectins, Western blots of the milk samples accord with ithe hemagglutination inhibition data and disclose the distribution of the human milk glycoproteins that inhibit each lectin. The data of this paper reveal the high efficiency of human milk components in blocking the P. aeruginosa lectins and the usefulness of these lectins for detecting milk glycoprotein saccharides, which may protect the infant against infections.
AB - The opportunistic human pathogen Pseudomonas aeruginosa produces a D-galactophilic (PA-IL) lectin and another lectin (PA-IIL) that binds L-fucose > D-arabinose > D-mannose in close association with its host-attacking factors. These lectins contribute to the virulence of P. aeruginosa by their involvement in the production, adhesion, and pathogenic effects of its biofilm on host cells. Therefore, they are considered targets for anti-Pseudomonas therapy. The present study compares their blocking by human milk samples with that of the plant lectin Con A. It demonstrates that human milk inhibits the hemagglutinating activities of the three lectins, with PA-IIL much more strongly inhibited than PA-IL or Con A. Using these lectins, Western blots of the milk samples accord with ithe hemagglutination inhibition data and disclose the distribution of the human milk glycoproteins that inhibit each lectin. The data of this paper reveal the high efficiency of human milk components in blocking the P. aeruginosa lectins and the usefulness of these lectins for detecting milk glycoprotein saccharides, which may protect the infant against infections.
KW - Glycoproteins
KW - Human milk
KW - Lectins
KW - Pseudomonas aeruginosa
KW - Western blotting
UR - http://www.scopus.com/inward/record.url?scp=0038209572&partnerID=8YFLogxK
U2 - 10.1139/w03-027
DO - 10.1139/w03-027
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C2 - 12795411
AN - SCOPUS:0038209572
SN - 0008-4166
VL - 49
SP - 230
EP - 235
JO - Canadian Journal of Microbiology
JF - Canadian Journal of Microbiology
IS - 3
ER -