Block in the elongation of protein synthesis in rabbit reticulocyte by action of the ionophore Valinomycin

Max Herzberg, Haim Breitbart

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

In this paper we show that ribosomes isolated from cells inhibited for protein synthesis by the antibiotic ionophore Valinomycin can still support incorporation of [14C]-leucine into polypeptides in a cell-free system. The extent of this functional integrity depends upon the concentration of Valinomycin used and whether it acted as an ionophore or not. We demonstrate that the antibiotic acts on protein synthesis at the level of elongation and has no action either at the initiation level or as activating an RNase. Moreover, we show that it can inhibit protein synthesis at concentrations where its action as an ionophore cannot be detected.

Original languageEnglish
Pages (from-to)163-167
Number of pages5
JournalMolecular Biology Reports
Volume6
Issue number3
DOIs
StatePublished - 31 Oct 1980

Fingerprint

Dive into the research topics of 'Block in the elongation of protein synthesis in rabbit reticulocyte by action of the ionophore Valinomycin'. Together they form a unique fingerprint.

Cite this