Abstract
Gelatin-modified poly(ethylene terephthalate) (PET) surfaces have been previously realized via an intermediate dopamine coating procedure that resulted in surfaces with superior haemocompatibility compared to unfunctionalized PET. The present study addresses the biocompatibility assessment of these coated PET surfaces. In this context, the stability of the gelatin coating upon exposure to physiological conditions and its cell-interactive properties were investigated. The proposed gelatin-dopamine-PET surfaces showed an increased protein coating stability up to 24 days and promoted the attachment and spreading of both endothelial cells (ECs) and smooth muscle cells (SMCs). In parallel, physisorbed gelatin coatings exhibited similar cell-interactive properties, albeit temporarily, as the coating delaminated within 1 week after cell seeding. Furthermore, no or only minimal immunogenic or inflammatory responses were observed during in vitro cytotoxicity and endotoxicity assessment for all gelatin-modified PET surfaces evaluated. Overall, the combined enhanced biocompatibility reported herein together with the previously proven haemocompatibility show the potential of the gelatin-dopamine-PET surfaces to function as vascular graft candidates.
| Original language | English |
|---|---|
| Pages (from-to) | 1258-1269 |
| Number of pages | 12 |
| Journal | Journal of Materials Chemistry B |
| Volume | 7 |
| Issue number | 8 |
| DOIs | |
| State | Published - 28 Feb 2019 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© The Royal Society of Chemistry.
Funding
The authors would like to thank UGent for the financial support under the form of the GOA project Biomedical Engineering for Improved Diagnosis and Patient-Tailored Treatment of Aortic Aneurysms and Dissection (BOF10/GOA/005). E. D. Giol acknowledges the Research Foundation Flanders for granting her an extended stay in Germany (FWO V418914N) that led to the herein presented cell studies and would like to thank Prof Karine Glinel and Prof. Alain Jonas for granting her a postdoctoral fellowship at UCL. Sandra Van Vlierberghe would like to acknowledge the Research Foundation Flanders (FWO, Belgium) for financial support under the form of research grants (FWOKN273, G005616N, G0F0516N).
| Funders | Funder number |
|---|---|
| University College London | |
| Fonds Wetenschappelijk Onderzoek | G0F0516N, FWOKN273, G005616N |