Biodistribution and function of coupled polymer-DNA origami nanostructures

Noah Joseph, Anastasia Shapiro, Ella Gillis, Shirin Barkey, Almogit Abu-Horowitz, Ido Bachelet, Boaz Mizrahi

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Spatial control over the distribution of therapeutics is a highly desired feature, which could limit the side effects of many drugs. Here we describe a nanoscale agent, fabricated from a coupled polymer-DNA origami hybrid that exhibits stability in serum and slow diffusion through tissues, in a manner correlating with shape and aspect ratio. Coupling to fragments of polyethylene glycol (PEG) through polyamine electrostatic interactions resulted in marked stability of the agents in-vivo, with > 90% of the agents maintaining structural integrity 5 days following subcutaneous injection. An agent functionalized with aptamers specific for human tumor necrosis factor TNF-alpha, significantly abrogated the inflammatory response in a delayed-type hypersensitivity model in humanized TNF-alpha mice. These findings highlight polymer-DNA hybrid nanostructures as a programmable and pharmacologically viable update to mainstream technologies such as monoclonal antibodies, capable of exerting an additional layer of control across the spatial dimension of drug activity.

Original languageEnglish
Article number19567
JournalScientific Reports
Issue number1
StatePublished - 10 Nov 2023
Externally publishedYes

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© 2023, The Author(s).


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