TY - JOUR
T1 - Biocompatible carbon dots derived from κ-carrageenan and phenyl boronic acid for dual modality sensing platform of sugar and its anti-diabetic drug release behavior
AU - Das, Poushali
AU - Maity, Priti Prasanna
AU - Ganguly, Sayan
AU - Ghosh, Sabyasachi
AU - Baral, Joydeep
AU - Bose, Madhuparna
AU - Choudhary, Sumita
AU - Gangopadhyay, Subhashis
AU - Dhara, Santanu
AU - Das, Amit Kumar
AU - Banerjee, Susanta
AU - Das, Narayan Chandra
N1 - Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - Detection of sugar by enzymatic assay has been suffering from costly, time-taking, instable and denaturation of glucose oxidase. Recently, chemosensors that have affinity towards boronate became the hot topic in the domain of monosaccharide detection. In this work, a novel strategy was addressed to fabricate carbon dots (C-dots) from linear sulfated polysaccharides κ- carrageenan and phenyl boronic acid for nonenzymatic monosaccharide (glucose) detection. The boronic acid group anchored C-dots surface can form assembly by covalently bonded with the cis-diol moiety of the glucose which caused fluorescence quenching of the C-dots. The inert surface nature of the luminescent C-dots enables them to sense as low as 1.7 μM glucose without the interference of other biomolecules. The proposed sensing system was successfully applied for assay of glucose in blood serum. Interestingly, these C-dots were used as a nano vehicle for delivery of anti-diabetic drug Metformin. Good biocompatibility results were found with MTT and hemolysis assay. Owing to its simplicity and effectiveness, the as-prepared C-dots offered great promise for blood sugar diagnosis and treatment.
AB - Detection of sugar by enzymatic assay has been suffering from costly, time-taking, instable and denaturation of glucose oxidase. Recently, chemosensors that have affinity towards boronate became the hot topic in the domain of monosaccharide detection. In this work, a novel strategy was addressed to fabricate carbon dots (C-dots) from linear sulfated polysaccharides κ- carrageenan and phenyl boronic acid for nonenzymatic monosaccharide (glucose) detection. The boronic acid group anchored C-dots surface can form assembly by covalently bonded with the cis-diol moiety of the glucose which caused fluorescence quenching of the C-dots. The inert surface nature of the luminescent C-dots enables them to sense as low as 1.7 μM glucose without the interference of other biomolecules. The proposed sensing system was successfully applied for assay of glucose in blood serum. Interestingly, these C-dots were used as a nano vehicle for delivery of anti-diabetic drug Metformin. Good biocompatibility results were found with MTT and hemolysis assay. Owing to its simplicity and effectiveness, the as-prepared C-dots offered great promise for blood sugar diagnosis and treatment.
KW - Carbon dots
KW - Drug delivery
KW - Glucose sensor
KW - Hemolysis
KW - Photoluminescence
UR - http://www.scopus.com/inward/record.url?scp=85063692056&partnerID=8YFLogxK
U2 - 10.1016/j.ijbiomac.2019.03.224
DO - 10.1016/j.ijbiomac.2019.03.224
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C2 - 30940582
AN - SCOPUS:85063692056
SN - 0141-8130
VL - 132
SP - 316
EP - 329
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -