Bioadhesive microneedle patches for tissue sealing

Eden Freundlich, Neta Shimony, Adi Gross, Boaz Mizrahi

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Sealing of soft tissues prevents leakage of gas and liquid, closes wounds, and promotes healing and is, therefore, of great significance in the clinical and medical fields. Although various formulations have been developed for reliable sealing of soft tissue, tradeoffs between adhesive properties, degradation profile, and tissue toxicity limit their clinical use. Hydrogel-based adhesives, for example, are highly biocompatible but adhere very weakly to the tissue and degrade quickly, while oxidized cellulose patches are poorly absorbed and may cause healing complications postoperatively. Here, we present a novel strategy for tissue sealing based on bioadhesive microneedle patches that can spontaneously adhere to tissue surface through electrostatic interactions and swell within it. A series of microneedle patches made of pullulan, chitosan, Carbopol, poly (lactic-co-glycolic acid), and a Carbopol/chitosan combination were fabricated and characterized for their use in tissue sealing. The effect of microneedle composition on the fabrication process, physical and mechanical properties, in vitro cytotoxicity, and in vivo biocompatibility were examined. The needle structure enables microneedles to strongly fix onto various tissues via physical interlocking, while their adhesive properties improve staying time and sealing capabilities. The microneedle patch comprising Carbopol needles and chitosan as a second pedestal layer presented the best results in terms of sealing and adhesion, a consequence of the needle's swelling and adhesion features combined with the supportive chitosan base layer. Finally, single Carbopol/chitosan patches stopped intense liver bleeding in a rat model significantly quicker and with less blood loss compared with commercial oxidized cellulose patches. These microneedles can be considered a promising cost-effective platform for adhering and sealing tissues as they can be applied quickly and painlessly, and require less trained medical staff and equipment.

Original languageEnglish
Article numbere10578
JournalBioengineering and Translational Medicine
Volume9
Issue number3
DOIs
StatePublished - May 2024
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.

Funding

This research was funded by the Israeli Ministry of Innovation, Science and Technology (Grant No. 0004497) and by the Russell Berrie Nanotechnology Institute, Technion, Israel Institute of Technology, Israel.

FundersFunder number
Israel Institute of Technology, Israel
Israeli Ministry of Innovation, Science and Technology0004497
Russell Berrie Nanotechnology Institute
Technion-Israel Institute of Technology

    Keywords

    • Carbopol
    • bioadhesive
    • biomaterials
    • chitosan
    • microneedle
    • sealant
    • tissue regeneration

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