TY - JOUR
T1 - Binding of Pseudomonas aeruginosa Lectin to Rhizobium sp.
AU - GILBOA‐GARBER, N.
AU - MIZRAHI, L.
PY - 1981/2
Y1 - 1981/2
N2 - Binding sites for the mannosephilic lectin of Pseudomonas aeruginosa and for concanavalin A were found on the nitrogen‐fixing symbiont Rhizobium sp. which infects Phaseolus lathyroides, and on the root cells of its host. The interaction between these lectins and the Rhizobium or the root cells was demonstrated by adsorption tests and by mannose‐specific peroxidase‐binding to the lectin‐coated bacteria. Treatment of the bacteria with formaldehyde, phenol, ethanol or boiling did not alter their ability to adsorb the lectins. The growth‐rate of the Rhizobium was unaffected when the Pseudomonas lectin was added to the culture medium. Addition of these lectins to the Rhizobium cells used for seedling infection led to an increased bacterial adsorption to seedling roots and to enlargement of nodule size, but did not increase nodulation.
AB - Binding sites for the mannosephilic lectin of Pseudomonas aeruginosa and for concanavalin A were found on the nitrogen‐fixing symbiont Rhizobium sp. which infects Phaseolus lathyroides, and on the root cells of its host. The interaction between these lectins and the Rhizobium or the root cells was demonstrated by adsorption tests and by mannose‐specific peroxidase‐binding to the lectin‐coated bacteria. Treatment of the bacteria with formaldehyde, phenol, ethanol or boiling did not alter their ability to adsorb the lectins. The growth‐rate of the Rhizobium was unaffected when the Pseudomonas lectin was added to the culture medium. Addition of these lectins to the Rhizobium cells used for seedling infection led to an increased bacterial adsorption to seedling roots and to enlargement of nodule size, but did not increase nodulation.
UR - http://www.scopus.com/inward/record.url?scp=0019364908&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2672.1981.tb00867.x
DO - 10.1111/j.1365-2672.1981.tb00867.x
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AN - SCOPUS:0019364908
SN - 0021-8847
VL - 50
SP - 21
EP - 28
JO - Journal of Applied Bacteriology
JF - Journal of Applied Bacteriology
IS - 1
ER -