Behaviour of crown gall tumor cells of sunflower with respect to the biosynthesis of ent-kaurene

Yedidya Gafni

doi:10.1016/0168-9452(89)90062-9

Behaviour of crown gall tumor cells of sunflower with respect to the biosynthesis of ent-kaurene

Yedidya Gafni

Agricultural Research Organization of Israel

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Biosynthesis of ent-kaurene was studied in extracts of germinating seeds and tumor cells of sunflower. Cell-free extracts derived from germinating seeds catalyzed the formation of [¹⁴C]ent-kaurene from [¹⁴C]mevalonate. On the other hand, extracts derived from tumor cells induced by two strains of Agrobacterium tumefaciens when incubated with [¹⁴C]mevalonate showed no conversion of the substrate to any detectable amount of [¹⁴C]ent-kaurene. Extracts of tumor cells were found to contain an inhibitory activity of ent-kaurene synthesis.

Original language	English
Pages (from-to)	171-175
Number of pages	5
Journal	Plant Science
Volume	65
Issue number	2
DOIs	https://doi.org/10.1016/0168-9452(89)90062-9
State	Published - 1989
Externally published	Yes

Keywords

Agrobacterium tumefaciens
Helianthus annuus
crown gall
ent-kaurene
gibberellin biosynthesis
sunflower

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10.1016/0168-9452(89)90062-9

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title = "Behaviour of crown gall tumor cells of sunflower with respect to the biosynthesis of ent-kaurene",

abstract = "Biosynthesis of ent-kaurene was studied in extracts of germinating seeds and tumor cells of sunflower. Cell-free extracts derived from germinating seeds catalyzed the formation of [14C]ent-kaurene from [14C]mevalonate. On the other hand, extracts derived from tumor cells induced by two strains of Agrobacterium tumefaciens when incubated with [14C]mevalonate showed no conversion of the substrate to any detectable amount of [14C]ent-kaurene. Extracts of tumor cells were found to contain an inhibitory activity of ent-kaurene synthesis.",

keywords = "Agrobacterium tumefaciens, Helianthus annuus, crown gall, ent-kaurene, gibberellin biosynthesis, sunflower",

author = "Yedidya Gafni",

year = "1989",

doi = "10.1016/0168-9452(89)90062-9",

language = "אנגלית",

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T1 - Behaviour of crown gall tumor cells of sunflower with respect to the biosynthesis of ent-kaurene

AU - Gafni, Yedidya

PY - 1989

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N2 - Biosynthesis of ent-kaurene was studied in extracts of germinating seeds and tumor cells of sunflower. Cell-free extracts derived from germinating seeds catalyzed the formation of [14C]ent-kaurene from [14C]mevalonate. On the other hand, extracts derived from tumor cells induced by two strains of Agrobacterium tumefaciens when incubated with [14C]mevalonate showed no conversion of the substrate to any detectable amount of [14C]ent-kaurene. Extracts of tumor cells were found to contain an inhibitory activity of ent-kaurene synthesis.

AB - Biosynthesis of ent-kaurene was studied in extracts of germinating seeds and tumor cells of sunflower. Cell-free extracts derived from germinating seeds catalyzed the formation of [14C]ent-kaurene from [14C]mevalonate. On the other hand, extracts derived from tumor cells induced by two strains of Agrobacterium tumefaciens when incubated with [14C]mevalonate showed no conversion of the substrate to any detectable amount of [14C]ent-kaurene. Extracts of tumor cells were found to contain an inhibitory activity of ent-kaurene synthesis.

KW - Agrobacterium tumefaciens

KW - Helianthus annuus

KW - crown gall

KW - ent-kaurene

KW - gibberellin biosynthesis

KW - sunflower

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VL - 65

SP - 171

EP - 175

JO - Plant Science

JF - Plant Science

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ER -

Behaviour of crown gall tumor cells of sunflower with respect to the biosynthesis of ent-kaurene

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