Behavioral circatidal rhythms require Bmal1 in Parhyale hawaiensis

Erica R. Kwiatkowski, Yisrael Schnytzer, Joshua J.C. Rosenthal, Patrick Emery

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Organisms living in the intertidal zone are exposed to a particularly challenging environment. In addition to daily changes in light intensity and seasonal changes in photoperiod and weather patterns, they experience dramatic oscillations in environmental conditions due to the tides. To anticipate tides, and thus optimize their behavior and physiology, animals occupying intertidal ecological niches have acquired circatidal clocks. Although the existence of these clocks has long been known, their underlying molecular components have proven difficult to identify, in large part because of the lack of an intertidal model organism amenable to genetic manipulation. In particular, the relationship between the circatidal and circadian molecular clocks, and the possibility of shared genetic components, has been a long-standing question. Here, we introduce the genetically tractable crustacean Parhyale hawaiensis as a system for the study of circatidal rhythms. First, we show that P. hawaiensis exhibits robust 12.4-h rhythms of locomotion that can be entrained to an artificial tidal regimen and are temperature compensated. Using CRISPR-Cas9 genome editing, we then demonstrate that the core circadian clock gene Bmal1 is required for circatidal rhythms. Our results thus demonstrate that Bmal1 is a molecular link between circatidal and circadian clocks and establish P. hawaiensis as a powerful system to study the molecular mechanisms underlying circatidal rhythms and their entrainment.

Original languageEnglish
Pages (from-to)1867-1882.e5
JournalCurrent Biology
Volume33
Issue number10
DOIs
StatePublished - 22 May 2023

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Inc.

Funding

We are grateful to N. Patel and his lab, in particular E. Alberstat at the MBL in Woods Hole for providing Parhyale hawaiensis colonies, and for their help and advice on CRISPR-Cas9 mutagenesis and animal care. We are additionally grateful to H. Melikian and her lab for their help and advice on genomic DNA extraction and cell culture experiments. This work was supported by a MIRA award from the National Institute of General Medicine Sciences ( 1R35GM118087 ) to P.E., a Whitman Research Fellowship from the Marine Biological Laboratory to P.E., and NSF grant # 1723141 to J.J.C.R., as well as NSF grant # 2139765 to P.E. and J.J.C.R.

FundersFunder number
National Science Foundation1723141, 2139765
National Institute of General Medical Sciences1R35GM118087
Marine Biological Laboratory

    Keywords

    • BMAL1
    • CRISPR-Cas9 mediated mutagenesis
    • Parhyale hawaiensis
    • circadian rhythms
    • circatidal rhythms

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