Bactericidal activity of the organo-tellurium compound AS101 against Enterobacter cloacae

Miriam Daniel-Hoffmann, Benjamin Sredni, Yeshayahu Nitzan

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25 Scopus citations

Abstract

Objectives: The antibacterial effect of the organo-tellurium compound AS101 on the Gram-negative bacterium Enterobacter cloacae is shown in this study for the first time. Methods: The antimicrobial effect of the drug was shown by inhibition of growth, by inhibition of biofilm formation and by its ability to penetrate the bacterial cell and to cause damage and ultrastructural changes. Results: AS101 was found to be a bactericidal drug with MICs and MBCs of 9.4 mg/L. It inhibits bacterial growth and causes a six orders of magnitude decrease in viability in a protein-rich medium, but not in a protein-poorer medium, unless 2-mercaptoethanol is added. Subinhibitory concentrations inhibit motility and biofilm formation. AS101 enters the bacterium through its porins and causes bacterial damage to Na. +/K. + pumps and leakage of potassium, phosphorous and sulphur. Ultrastructural changes within the bacterial cell and on its surface demonstrate an incomplete surface with a concavity in the centre that looks like a hole from which aggregates are liberated as well as cell lysis. Conclusions: AS101 has antibacterial activity, which may be useful against E. cloacae and other species of Enterobacteriaceae as a substitute for current antibiotics that have become ineffective due to increasing bacterial resistance.

Original languageEnglish
Article numberdks185
Pages (from-to)2165-2172
Number of pages8
JournalJournal of Antimicrobial Chemotherapy
Volume67
Issue number9
DOIs
StatePublished - Sep 2012

Bibliographical note

Funding Information:
This work was partly supported by the Dr Tovi Comet-Walerstein Cancer Research Chair, the Dave and Florence Muskovitz Chair in Cancer Research and the Frieda Stollman Cancer Memorial Fund. This research was also supported in part by the Rappaport Foundation for Microbiology, Bar-Ilan University, Israel (to Y. N.).

Funding

This work was partly supported by the Dr Tovi Comet-Walerstein Cancer Research Chair, the Dave and Florence Muskovitz Chair in Cancer Research and the Frieda Stollman Cancer Memorial Fund. This research was also supported in part by the Rappaport Foundation for Microbiology, Bar-Ilan University, Israel (to Y. N.).

FundersFunder number
Frieda Stollman Cancer Memorial Fund
Rappaport Foundation for Microbiology, Bar-Ilan University, Israel

    Keywords

    • Antimicrobial activity
    • Motility and biofilm formation inhibition
    • Ultrastructural changes

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