Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation

Avihai Perl, Shmuel Galili, Orit Shaul, Inbal Ben-Tzvi, Gad Galili

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.

Original languageEnglish
Pages (from-to)715-718
Number of pages4
JournalNature Biotechnology
Volume11
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Dive into the research topics of 'Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation'. Together they form a unique fingerprint.

Cite this