TY - JOUR
T1 - Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase
T2 - Two novel selectable markers for plant transformation
AU - Perl, Avihai
AU - Galili, Shmuel
AU - Shaul, Orit
AU - Ben-Tzvi, Inbal
AU - Galili, Gad
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1993
Y1 - 1993
N2 - Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.
AB - Potato plants transformed with a chimeric gene encoding a bacterial desensitized aspartate kinase were selected for resistance to the presence of lysine plus threonine in the regeneration and rooting media. Similarly, plants transformed with a chimeric gene encoding a bacterial dihydrodipicolinate synthase were selected for resistance to the toxic lysine analog S-aminoethyl L-cysteine. In both cases, resistant plants were regenerated, and all were apparently transgenic based on their content of dihydrodipicolinate synthase or aspartate kinase activities that were significantly higher, and much less sensitive to lysine and threonine inhibition, than the endogenous activities in control untransformed plants. Our data suggest that these novel selectable markers may be useful for the isolation of transgenic plants expressing relatively high levels of the gene of interest.
UR - http://www.scopus.com/inward/record.url?scp=0027238216&partnerID=8YFLogxK
U2 - 10.1038/nbt0693-715
DO - 10.1038/nbt0693-715
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SN - 1087-0156
VL - 11
SP - 715
EP - 718
JO - Nature Biotechnology
JF - Nature Biotechnology
IS - 6
ER -