B cells populating the multiple sclerosis brain mature in the draining cervical lymph nodes

Joel N.H. Stern, Gur Yaari, Jason A. Vander Heiden, George Church, William F. Donahue, Rogier Q. Hintzen, Anita J. Huttner, Jon D. Laman, Rashed M. Nagra, Alyssa Nylander, David Pitt, Sriram Ramanan, Bilal A. Siddiqui, Francois Vigneault, Steven H. Kleinstein, David A. Hafler, Kevin C. O'Connor

Research output: Contribution to journalArticlepeer-review

336 Scopus citations

Abstract

Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) characterized by autoimmunemediated demyelination and neurodegeneration. The CNS of patients withMSharbors expanded clones of antigen-experienced B cells that reside in distinct compartments including the meninges, cerebrospinal fluid (CSF), and parenchyma. It is not understood whether this immune infiltrate initiates its development in the CNS or in peripheral tissues. B cells in the CSF can exchange with those in peripheral blood, implying that CNS B cells may have access to lymphoid tissue that may be the specific compartment(s) in which CNS-resident B cells encounter antigen and experience affinitymaturation. Paired tissues were used to determine whether the B cells that populate the CNS mature in the draining cervical lymph nodes (CLNs). High-throughput sequencing of the antibody repertoire demonstrated that clonally expanded B cells were present in both compartments. Founding members of clones were more often found in the draining CLNs. More mature clonal members derived from these founders were observed in the draining CLNs and also in the CNS, including lesions. These data provide new evidence that B cells traffic freely across the tissue barrier, with the majority of B cell maturation occurring outside of the CNS in the secondary lymphoid tissue. Our study may aid in further defining the mechanisms of immunomodulatory therapies that either deplete circulating B cells or affect the intrathecal B cell compartment by inhibiting lymphocyte transmigration into the CNS.

Original languageEnglish
Article number248ra107
JournalScience Translational Medicine
Volume6
Issue number248
DOIs
StatePublished - 6 Aug 2014

Funding

FundersFunder number
National Institute of Mental Health
National Institute of Neurological Disorders and Stroke
National Institutes of HealthU19 AI070352, RR19895, P01 AI039671, T15 LM07056, U19AI050864, TG T32GM07205, T32 8507596, U19 AI046130, P01 AI045757, R01AI104739
National Multiple Sclerosis Society
National Institute of Allergy and Infectious DiseasesU19AI046130

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