Abstract
B lymphopoiesis has historically been depicted as a unidirectional process, in which cohorts of developing cells transit through successive differentiative stages in an irreversible, synchronous manner. Here, we examine this view by combining kinetic analysis of developing B cell subsets in the bone marrow with mathematical modeling. Our bromo-deoxyuridine (BrdU) labeling data are incompatible with B cell development being a synchronous process, because labeling curves are non-linear. Moreover, we show that B cell development may not be completely unidirectional, because our results support the possibility of a phenotypic 'reflux' among the immature to the pre-B cell subsets.
| Original language | English |
|---|---|
| Pages (from-to) | 301-312 |
| Number of pages | 12 |
| Journal | International Immunology |
| Volume | 15 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1 Mar 2003 |
Bibliographical note
Funding Information:We are grateful to M. Gorfine and L. Freedman for the derivation of the fitting criterion and likelihood ratio estimates for two interdependent populations. Supported in part by Israel Science Foundation grant 759/01-1, the Yigal Alon Fellowship and a Bar-Ilan University internal grant (to R. M.).
Funding
We are grateful to M. Gorfine and L. Freedman for the derivation of the fitting criterion and likelihood ratio estimates for two interdependent populations. Supported in part by Israel Science Foundation grant 759/01-1, the Yigal Alon Fellowship and a Bar-Ilan University internal grant (to R. M.).
| Funders | Funder number |
|---|---|
| Yigal Alon Fellowship | |
| Bar-Ilan University | |
| Israel Science Foundation | 759/01-1 |
Keywords
- B lymphocyte subsets
- Bromo-deoxyuridine
- Mathematical model