TY - JOUR
T1 - Arsenic Trioxide inhibits the growth of human ovarian carcinoma cell line
AU - Bornstein, Jacob
AU - Sagi, Shlomi
AU - Haj, Amer
AU - Harroch, Jeffrey
AU - Fares, Fuad
PY - 2005/12
Y1 - 2005/12
N2 - Objective. To determine whether Arsenic Trioxide may be an effective treatment of human ovarian carcinoma, we examined if it induced apoptosis in human ovarian cancer cell line. Methods. The human ovarian cell line SKOV3 was exposed to different concentrations of As2O3: 0 (control), 0.1 μM, 1 μM, 5 μM and 10 μM. The effect on cells proliferation and apoptotic parameters was examined. Results. The most effective inhibitory Arsenic Trioxide concentrations were 5 μM and 10 μM, causing growth inhibition of 79% and 83%, respectively. The maximum inhibitory effect of Arsenic Trioxide on cellular proliferation was seen after 48 h. No morphological or molecular features of apoptosis have been observed. At the same time, there were no typical changes of cellular necrosis. Conclusions. The exact mechanism by which Arsenic Trioxide inhibits the growth of ovarian cancer cells is probably not by apoptosis. However, since As2O3 has been shown to inhibit ovarian cancer cellular growth, it should be further elucidated as a possible chemotherapeutic agent of ovarian cancer.
AB - Objective. To determine whether Arsenic Trioxide may be an effective treatment of human ovarian carcinoma, we examined if it induced apoptosis in human ovarian cancer cell line. Methods. The human ovarian cell line SKOV3 was exposed to different concentrations of As2O3: 0 (control), 0.1 μM, 1 μM, 5 μM and 10 μM. The effect on cells proliferation and apoptotic parameters was examined. Results. The most effective inhibitory Arsenic Trioxide concentrations were 5 μM and 10 μM, causing growth inhibition of 79% and 83%, respectively. The maximum inhibitory effect of Arsenic Trioxide on cellular proliferation was seen after 48 h. No morphological or molecular features of apoptosis have been observed. At the same time, there were no typical changes of cellular necrosis. Conclusions. The exact mechanism by which Arsenic Trioxide inhibits the growth of ovarian cancer cells is probably not by apoptosis. However, since As2O3 has been shown to inhibit ovarian cancer cellular growth, it should be further elucidated as a possible chemotherapeutic agent of ovarian cancer.
KW - Chemotherapy
KW - Cytotoxic agent
KW - Female
KW - Human neoplasms
UR - http://www.scopus.com/inward/record.url?scp=28044460481&partnerID=8YFLogxK
U2 - 10.1016/j.ygyno.2005.07.125
DO - 10.1016/j.ygyno.2005.07.125
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C2 - 16243384
AN - SCOPUS:28044460481
SN - 0090-8258
VL - 99
SP - 726
EP - 729
JO - Gynecologic Oncology
JF - Gynecologic Oncology
IS - 3
ER -
