Application of a solid membrane ion-selective electrode to in vivo measurements

A solid membrane electrode containing a highly selective ion-complexing agent immobilized in a polymer membrane is applied to measurements in vivo from the surface of an organ. For the measurements reported here, an ion-complexing agent selective for potassium is incorporated in a silicone rubber membrane and applied to measure ion changes in the brain. Comparison is made to the measurements recorded with an ion-selective microelectrode in the cortical tissue. The surface electrode compares favorably with the microelectrode in terms of amplitude of response and response time for the changes Leao. The methods reported here are amenable to a selection of ion measurements by incorporation of a suitable ion exchanger in a polymer membrane. Extracellular ion activity is monitored in the anesthetized animal by holding the electrode in a balanced suspension which provides a light, flexible contact to the organ of interest. In the unanesthetized animal, when recording from the brain, the electrode is fixed in a skull-implanted cannula.