TY - JOUR
T1 - Antimicrobial susceptibility of Clostridium difficile isolates in Israel
AU - Tkhawkho, Linda
AU - Nitzan, Orna
AU - Pastukh, Nina
AU - Brodsky, Diana
AU - Jackson, Karen
AU - Peretz, Avi
N1 - Publisher Copyright:
© 2017 International Society for Chemotherapy of Infection and Cancer
PY - 2017/9
Y1 - 2017/9
N2 - Objectives An increase of Clostridium difficile isolates with reduced susceptibility to various antimicrobial agents has been observed, including isolates that are non-susceptible to antibiotics that are routinely used for treatment of C. difficile, such as vancomycin and metronidazole. We determined the susceptibility rates of C. difficile isolates from hospitals in northern Israel to various antibiotics including tigecycline, which was not previously reported from Israel. Methods A total of 81 stool samples were collected from three hospitals in northern Israel from patients with C. difficile infection. Specimens were screened for BI/NAP1/027 ribotype, cultured, and sensitivity tests were performed for vancomycin, metronidazole, moxifloxacin, and tigecycline. Statistical tests were applied for analysing the differences in distribution of resistance between the different antibiotics and between BI/NAP1/027 and resistance of antibiotics. Results Reduced susceptibility was found among 6/81 isolates for vancomycin, 4/81 for metronidazole, and 17/81 for moxifloxacin. Only 1 isolate had reduced susceptibility to tigecycline, with a mean MIC of 0.05 μg/mL. Reduced susceptibility to moxifloxacin was significantly associated with reduced susceptibility to vancomycin (p = 0.016) and to metronidazole (p = 0.0276), and reduced susceptibility to metronidazole was associated with reduced susceptibility to vancomycin (p = 0.0259). Eight of 81 isolates (9.9%) were positive for BI/NAP1/027 ribotype and had significantly higher non-susceptibility rates to moxifloxacin and vancomycin compared with BI/NAP1/027 negative isolates (p < 0.0001 and p = 0.0113, respectively). Conclusions We found higher non-susceptibility rates to vancomycin and metronidazole than most previous studies, while tigecycline resistance rates are very low in northern Israel, rendering it a potential agent for treating CDI.
AB - Objectives An increase of Clostridium difficile isolates with reduced susceptibility to various antimicrobial agents has been observed, including isolates that are non-susceptible to antibiotics that are routinely used for treatment of C. difficile, such as vancomycin and metronidazole. We determined the susceptibility rates of C. difficile isolates from hospitals in northern Israel to various antibiotics including tigecycline, which was not previously reported from Israel. Methods A total of 81 stool samples were collected from three hospitals in northern Israel from patients with C. difficile infection. Specimens were screened for BI/NAP1/027 ribotype, cultured, and sensitivity tests were performed for vancomycin, metronidazole, moxifloxacin, and tigecycline. Statistical tests were applied for analysing the differences in distribution of resistance between the different antibiotics and between BI/NAP1/027 and resistance of antibiotics. Results Reduced susceptibility was found among 6/81 isolates for vancomycin, 4/81 for metronidazole, and 17/81 for moxifloxacin. Only 1 isolate had reduced susceptibility to tigecycline, with a mean MIC of 0.05 μg/mL. Reduced susceptibility to moxifloxacin was significantly associated with reduced susceptibility to vancomycin (p = 0.016) and to metronidazole (p = 0.0276), and reduced susceptibility to metronidazole was associated with reduced susceptibility to vancomycin (p = 0.0259). Eight of 81 isolates (9.9%) were positive for BI/NAP1/027 ribotype and had significantly higher non-susceptibility rates to moxifloxacin and vancomycin compared with BI/NAP1/027 negative isolates (p < 0.0001 and p = 0.0113, respectively). Conclusions We found higher non-susceptibility rates to vancomycin and metronidazole than most previous studies, while tigecycline resistance rates are very low in northern Israel, rendering it a potential agent for treating CDI.
KW - BI/NAP1/027
KW - Clostridium difficile
KW - Culture
KW - Metronidazole
KW - Moxifloxacin
KW - Tigecycline
KW - Vancomycin
UR - http://www.scopus.com/inward/record.url?scp=85026370392&partnerID=8YFLogxK
U2 - 10.1016/j.jgar.2017.04.005
DO - 10.1016/j.jgar.2017.04.005
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C2 - 28729205
AN - SCOPUS:85026370392
SN - 2213-7165
VL - 10
SP - 161
EP - 164
JO - Journal of Global Antimicrobial Resistance
JF - Journal of Global Antimicrobial Resistance
ER -