Antimicrobial sensitivity studies in gastrointestinal diseases in bovines

Thirty carcasses of bovines received for necropsy at the postmortem hall of the Department of Veterinary Pathology, LLRUVAS, Hisar. Cultural isolates from liver, intestinal contents, abomasum, mesenteric lymph nodes, spleen, heart, lungs and kidneys were subjected to antimicrobial sensitivity testing using 20 antimicrobials of standard concentrations by the single disc diffusion method. The E. coli isolates were found to be most sensitive to amikacin (90 per cent) followed by polymixin B (85 per cent), colistin and gentamicin (80 per cent). Maximum resistance was observed to cotrimoxazole, amoxycillin, oxytetracycline and amoxycillin+sulbactum (85 per cent). Salmonella spp. was found highly sensitive to amikacin, colistin and ofloxacin (100 per cent) and resistant to ampicillin and amoxycillin+sulbactum (100 per cent). The sensitivity pattern of Klebsiella spp. revealed maximum sensitivity to gentamicin and, furoxone (100 per cent), whereas, they were found resistant to cefoperazone (100 per cent). Pseudomonas spp. was found most sensitive to amikacin, colistin, and enrofloxacin (100 per cent) followed by cotrimoxazole (80 per cent). It was found resistant to ampicillin, amoxycillin, doxycycline hydrochloride, amoxyclav, oxytetracycline, amoxycillin+sulbactum and ofloxacin (100 per cent). Staphylococcus spp. was most sensitive to amikacin, doxycycline hydrochloride, furoxone, amoxyclav and ofloxacin (100 per cent) followed by polymixinB (75 per cent) and cefoperazone (65 per cent) whereas, they were found most resistant to ampicillin, amoxicillin, nalidixic acid, oxytetracycline and chloramphenicol (100 per cent). Streptococcus spp. was most sensitive to amoxyclav and amoxycillin+sulbactum (100 per cent) followed by oxytetracycline (85 per cent) and doxycycline hydrochloride (80 per cent) whereas, they were found most resistant to colistin, nalidixic acid, cefoperazone, cotrimoxazole, gentamicin, enrofloxacin and chloramphenicol (100 per cent).