TY - JOUR
T1 - Antigenic variability of foot-and-mouth disease virus serotype O during serial cytolytic passage
AU - Subramaniam, Saravanan
AU - Das, Biswajit
AU - Biswal, Jitendra K.
AU - Ranjan, Rajeev
AU - Pattnaik, Bramhadev
N1 - Publisher Copyright:
© 2017, Springer Science+Business Media, LLC.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationship value <0.3) with the serotype O vaccine strain and upon serial passage increase in relationship value was observed. Comparison of capsid sequence revealed substitution at four positions (VP3:K58 → E and P158 → S, VP1:E83 → K and R172 → Q) acquired during the serial passage. Examination of passage level and amino acid substitution revealed the critical role of position VP3-58 that was identified earlier as crucial for antigenic site IV, in the observed antigenic variability. The role of position VP3-58 was further confirmed using reverse genetics approach.
AB - The emergence and disappearance of antigenic variants of foot-and-mouth disease virus (FMDV) during a field outbreak occurs periodically due to the volatile nature of its genome. In the present analysis, change in antigenic behavior of serotype O FMDV during the serial cytolytic passage in the absence of immune pressure was observed. Initially, the isolate showed a poor antigenic match (relationship value <0.3) with the serotype O vaccine strain and upon serial passage increase in relationship value was observed. Comparison of capsid sequence revealed substitution at four positions (VP3:K58 → E and P158 → S, VP1:E83 → K and R172 → Q) acquired during the serial passage. Examination of passage level and amino acid substitution revealed the critical role of position VP3-58 that was identified earlier as crucial for antigenic site IV, in the observed antigenic variability. The role of position VP3-58 was further confirmed using reverse genetics approach.
KW - Antigenic variability
KW - Cytolytic passage
KW - FMDV
KW - Serotype O
KW - Site IV
UR - http://www.scopus.com/inward/record.url?scp=85024491364&partnerID=8YFLogxK
U2 - 10.1007/s11262-017-1494-3
DO - 10.1007/s11262-017-1494-3
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C2 - 28718047
AN - SCOPUS:85024491364
SN - 0920-8569
VL - 53
SP - 931
EP - 934
JO - Virus Genes
JF - Virus Genes
IS - 6
ER -
