Angiotensin II induces acrosomal exocytosis in bovine spermatozoa

Ejaculated mammalian spermatozoa must reside in the female genital tract for some time before gaining the ability to fertilize the egg. During this time, spermatozoa undergo some physiological changes that collectively are called capacitation. Capacitation of mammalian spermatozoa is a prerequisite for acrosome reaction, which is an exocytotic event occurring before fertilization. The specific biophysical and biochemical changes that accompany sperm capacitation and the agonists inducing acrosome reaction are not fully understood. Using SDS-gel electrophoresis and immunoblotting, we demonstrate the existence of a class of angiotensin receptors (AT₁) in bovine spermatozoa. In capacitated sperm, we show that angiotensin II (ANG II) AT₁ receptors are localized in the head and tail, whereas in noncapacitated cells the receptors are localized in the tail only. We find that ANG II markedly stimulates acrosomal exocytosis of capacitated bovine spermatozoa in vitro in a concentration range of 0.1-10 nM. No effect of ANG II was found in noncapacitated cells. The ability of ANG II to stimulate the acrosome reaction depends on the presence of calcium ions in the incubation medium. The ANG II-induced acrosome reaction was markedly inhibited by a selective AT₁ receptor antagonist, losartan (DUP 753). PD-123319, a selective antagonist of the ANG II AT₂ receptor, had no effect on the ANG II-induced acrosome reaction. Thus ANG II via activation of AT₁ receptors may play a regulatory role in the induction of the acrosome reaction.