Ancient DNA analysis of 101 cattle remains: Limits and prospects

Ceiridwen J. Edwards; David E. MacHugh; Keith M. Dobney; Louise Martin; Nerissa Russell; Liora K. Horwitz; Susan K. McIntosh; Kevin C. MacDonald; Daniel Helmer; Anne Tresset; Jean Denis Vigne; Daniel G. Bradley

doi:10.1016/j.jas.2003.11.001

Ancient DNA analysis of 101 cattle remains: Limits and prospects

Ceiridwen J. Edwards, David E. MacHugh, Keith M. Dobney, Louise Martin, Nerissa Russell, Liora K. Horwitz, Susan K. McIntosh, Kevin C. MacDonald, Daniel Helmer, Anne Tresset, Jean Denis Vigne, Daniel G. Bradley

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

A total of 101 cattle teeth and bones from 13 archaeological sites between 1000 to 9000 years old were assessed for the presence of verifiable mitochondrial sequences. It was possible to reproducibly amplify and sequence mitochondrial control region DNA extracted from twelve of the samples. The results were compared with published extant data by constructing phylogenetic networks. The sequences obtained from the cattle specimens were either identical to the reference sequence for modern cattle or closely related to it. They included three sequences not previously documented. The network analysis of the ancient data highlights the proximity of the ancient DNA cattle sequences to modern Near Eastern, European and African Bos taurus, as well as regional continuity.

Original language	English
Pages (from-to)	695-710
Number of pages	16
Journal	Journal of Archaeological Science
Volume	31
Issue number	6
DOIs	https://doi.org/10.1016/j.jas.2003.11.001
State	Published - Jun 2004
Externally published	Yes

Funding

We thank Andrew Chamberlain for the CPC wild boar and aurochs specimens, and Yvonne Edwards for commenting on the manuscript. Authorisation for sampling from the various sites was from the following: E. Galili, N. Goring-Morris, H. Khalaily and O. Marder (Israel), J.-L. Roudil (Ardèche), and R. J. McIntosh (Africa). C. J. Edwards is supported by the Enterprise Ireland Basic Research Grants Scheme (project no. SC/1999/409). This work was part-funded by The Wellcome Trust (grant no. 047485/Z/96/Z), and a Eurocores OMLL Programme Grant via CNRS, France.

Funders	Funder number
Enterprise Ireland Basic Research Grants Scheme	SC/1999/409
Wellcome Trust	047485/Z/96/Z
Centre National de la Recherche Scientifique

Keywords

Ancient DNA
Biomolecular archaeology
Cattle
Mitochondrial haplotypes
Polymerase chain reaction

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10.1016/j.jas.2003.11.001

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title = "Ancient DNA analysis of 101 cattle remains: Limits and prospects",

abstract = "A total of 101 cattle teeth and bones from 13 archaeological sites between 1000 to 9000 years old were assessed for the presence of verifiable mitochondrial sequences. It was possible to reproducibly amplify and sequence mitochondrial control region DNA extracted from twelve of the samples. The results were compared with published extant data by constructing phylogenetic networks. The sequences obtained from the cattle specimens were either identical to the reference sequence for modern cattle or closely related to it. They included three sequences not previously documented. The network analysis of the ancient data highlights the proximity of the ancient DNA cattle sequences to modern Near Eastern, European and African Bos taurus, as well as regional continuity.",

keywords = "Ancient DNA, Biomolecular archaeology, Cattle, Mitochondrial haplotypes, Polymerase chain reaction",

author = "Edwards, {Ceiridwen J.} and MacHugh, {David E.} and Dobney, {Keith M.} and Louise Martin and Nerissa Russell and Horwitz, {Liora K.} and McIntosh, {Susan K.} and MacDonald, {Kevin C.} and Daniel Helmer and Anne Tresset and Vigne, {Jean Denis} and Bradley, {Daniel G.}",

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AU - Edwards, Ceiridwen J.

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AU - Dobney, Keith M.

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AU - Russell, Nerissa

AU - Horwitz, Liora K.

AU - McIntosh, Susan K.

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AU - Tresset, Anne

AU - Vigne, Jean Denis

AU - Bradley, Daniel G.

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AB - A total of 101 cattle teeth and bones from 13 archaeological sites between 1000 to 9000 years old were assessed for the presence of verifiable mitochondrial sequences. It was possible to reproducibly amplify and sequence mitochondrial control region DNA extracted from twelve of the samples. The results were compared with published extant data by constructing phylogenetic networks. The sequences obtained from the cattle specimens were either identical to the reference sequence for modern cattle or closely related to it. They included three sequences not previously documented. The network analysis of the ancient data highlights the proximity of the ancient DNA cattle sequences to modern Near Eastern, European and African Bos taurus, as well as regional continuity.

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KW - Biomolecular archaeology

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Ancient DNA analysis of 101 cattle remains: Limits and prospects

Abstract

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Keywords

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