Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43

Xue Hai Liang; Qing Liu; Li Liu; Christian Tschudi; Shulamit Michaeli

doi:10.1016/j.molbiopara.2005.09.004

Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43

Xue Hai Liang
, Qing Liu
, Li Liu
, Christian Tschudi
, Shulamit Michaeli

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

In trypanosomatids all mRNAs undergo trans-splicing, whereas cis-splicing is restricted to a few transcripts. Trans-splicing is mechanistically similar to cis-splicing, however, little is known about the trans-splicing machinery and its underlying mechanism. In this study, we examined the involvement of splicing factors in cis- and trans-splicing by RNA interference (RNAi). Two factors (Prp31 and Prp43) were found to be essential for both pathways, suggesting that splicing factors are shared by these two reactions. We identified a 45S complex carrying pre-mRNA and all the U-snRNAs, including U1 and the SL RNA, suggesting that a single spliceosomal complex may potentially conduct both trans- and cis-splicing.

Original language	English
Pages (from-to)	29-39
Number of pages	11
Journal	Molecular and Biochemical Parasitology
Volume	145
Issue number	1
DOIs	https://doi.org/10.1016/j.molbiopara.2005.09.004
State	Published - Jan 2006

Bibliographical note

Funding Information:
The research was supported in part by a grant from the German Israeli Foundation (to S.M.) and the USA-Binational Foundation grant to S.M. and in part by National Institutes of Health Grant AI28798 (to Elisabetta Ullu). S.M. is an International Scholar of the Howard Hughes Medical Institute. C.T. was the recipient of a Burroughs Wellcome Fund New Investigator Award in Molecular Parasitology.

Funding

The research was supported in part by a grant from the German Israeli Foundation (to S.M.) and the USA-Binational Foundation grant to S.M. and in part by National Institutes of Health Grant AI28798 (to Elisabetta Ullu). S.M. is an International Scholar of the Howard Hughes Medical Institute. C.T. was the recipient of a Burroughs Wellcome Fund New Investigator Award in Molecular Parasitology.

Funders	Funder number
German Israeli Foundation
USA-Binational Foundation
National Institutes of Health
National Institute of Allergy and Infectious Diseases	R37AI028798

Keywords

Spliceosome
Trans-splicing
Trypanosome
U-snRNP

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10.1016/j.molbiopara.2005.09.004

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title = "Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43",

abstract = "In trypanosomatids all mRNAs undergo trans-splicing, whereas cis-splicing is restricted to a few transcripts. Trans-splicing is mechanistically similar to cis-splicing, however, little is known about the trans-splicing machinery and its underlying mechanism. In this study, we examined the involvement of splicing factors in cis- and trans-splicing by RNA interference (RNAi). Two factors (Prp31 and Prp43) were found to be essential for both pathways, suggesting that splicing factors are shared by these two reactions. We identified a 45S complex carrying pre-mRNA and all the U-snRNAs, including U1 and the SL RNA, suggesting that a single spliceosomal complex may potentially conduct both trans- and cis-splicing.",

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Analysis of spliceosomal complexes in Trypanosoma brucei and silencing of two splicing factors Prp31 and Prp43

Abstract

Bibliographical note

Funding

Keywords

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