Analyses of diverse mammals' milk and lactoferrin glycans using five pathogenic bacterial lectins

Keren D. Zinger-Yosovich, Dvora Sudakevitz, David Iluz, Nechama Gilboa-Garber

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Milk indigested glycans hamper infections by blocking pathogen adhesion to babies' cells via lectins (sugar-binding proteins). This study describes usage of five pathogenic bacterial lectins and two plant lectins for analyses of alpaca, buffalo, camel, cow, dog, fallow deer, giraffe, goat, horse, human, rabbit, and sheep milks, and also commercial human and cow milk lactoferrins. The lectins used differentially reacted with the 12 milks - most strongly with humans' ones. Most of them (excluding Pseudomonas aeruginosa galactophilic PA-IL) were also sensitive to the human and cow lactoferrins. The fucophilic bacterial lectins were most sensitive to human lactoferrin, while the mannophilic ones - to the cow's. The actual function of bacterial lectins in pathogen adhesion and their non-glycosylated structures (evading non-specific interactions) are advantageous for such studies. This study shows the efficiency of the bacterial lectins for milk analyses: differentiating between the diverse milks, estimating their anti-infection potentials, and probing their active glycans.

Original languageEnglish
Pages (from-to)1335-1342
Number of pages8
JournalFood Chemistry
Issue number4
StatePublished - Feb 2011


  • Glycans
  • Lactoferrins
  • Lectin blocking
  • Milks
  • Pathogenic bacterial lectins
  • Western blotting


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