An ultra-sensitive dual-mode imaging system using metal-enhanced fluorescence in solid phantoms

Eran A. Barnoy, Dror Fixler, Rachela Popovtzer, Tsviya Nayhoz, Krishanu Ray

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In this study, we developed a highly sensitive dual-mode imaging system using gold nanoparticles (GNPs) conjugated to various fluorophores in solid phantoms. The system consists of fluorescence-lifetime imaging microscopy (FLIM) for surface imaging, diffusion reflection (DR) for deep-tissue imaging (up to 1 cm), and metal-enhanced fluorescence (MEF). We detected quenching in the fluorescent intensity (FI) for the conjugation of both gold nanospheres (GNS) and gold nanorods (GNRs) to Fluorescein, which has an excitation peak at a wavelength shorter than the surface plasmon resonance (SPR) of both types of GNPs. Enhanced FI was detected in conjugation to Rhodamine B (RhB) and Sulforhodamine B (SRB), both with excitation peaks in the SPR regions of the GNPs. The enhanced FI was detected both in solution and in solid phantoms by the FLIM measurements. DR measurements detected the presence of GNRs within the solid phantoms by recording the dropped rates of light scattering in wavelengths corresponding to the absorption spectra of the GNRs. With the inclusion of MEF, this promising dual-mode imaging technique enables efficient and sensitive molecular and functional imaging. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)3912-3921
Number of pages10
JournalNano Research
Issue number12
StatePublished - 1 Dec 2015

Bibliographical note

Publisher Copyright:
© 2015, Tsinghua University Press and Springer-Verlag Berlin Heidelberg.


  • biomolecular imaging
  • diffusion reflection
  • fluorescence lifetime imaging
  • gold nanoparticles
  • metal-enhanced fluorescence
  • noninvasive detection


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