TY - JOUR
T1 - An mRNA structure that controls gene expression by binding FMN
AU - Winkler, Wade C.
AU - Cohen-Chalamish, Smadar
AU - Breaker, Ronald R.
PY - 2002/12/10
Y1 - 2002/12/10
N2 - The RFN element is a highly conserved domain that is found frequently in the 5′-untranslated regions of prokaryotic mRNAS that encode for flavin mononucleotide (FMN) biosynthesis and transport proteins. We report that this domain serves as the receptor for a metabolite-dependent riboswitch that directly binds FMN in the absence of proteins. Our results also indicate that in Bacillus subtilis, the riboswitch most likely controls gene expression by causing premature transcription termination of the rib-DEAHT operon and precluding access to the ribosome-binding site of ypaA mRNA. Sequence and structural analyses indicate that the RFN element is a natural FMN-binding aptamer, the allosteric character of which is harnessed to control gene expression.
AB - The RFN element is a highly conserved domain that is found frequently in the 5′-untranslated regions of prokaryotic mRNAS that encode for flavin mononucleotide (FMN) biosynthesis and transport proteins. We report that this domain serves as the receptor for a metabolite-dependent riboswitch that directly binds FMN in the absence of proteins. Our results also indicate that in Bacillus subtilis, the riboswitch most likely controls gene expression by causing premature transcription termination of the rib-DEAHT operon and precluding access to the ribosome-binding site of ypaA mRNA. Sequence and structural analyses indicate that the RFN element is a natural FMN-binding aptamer, the allosteric character of which is harnessed to control gene expression.
UR - http://www.scopus.com/inward/record.url?scp=0037058920&partnerID=8YFLogxK
U2 - 10.1073/pnas.212628899
DO - 10.1073/pnas.212628899
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C2 - 12456892
AN - SCOPUS:0037058920
SN - 0027-8424
VL - 99
SP - 15908
EP - 15913
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 25
ER -