An in vitro microfluidic approach to generating protein-interaction networks

Doron Gerber; Sebastian J. Maerkl; Stephen R. Quake

doi:10.1038/nmeth.1289

An in vitro microfluidic approach to generating protein-interaction networks

Doron Gerber, Sebastian J. Maerkl, Stephen R. Quake

Research output: Contribution to journal › Article › peer-review

130 Scopus citations

Abstract

We developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways.

Original language	English
Pages (from-to)	71-74
Number of pages	4
Journal	Nature Methods
Volume	6
Issue number	1
DOIs	https://doi.org/10.1038/nmeth.1289
State	Published - Jan 2009
Externally published	Yes

Bibliographical note

Funding Information:
We thank members of the Stanford microfluidics foundry for help with device fabrication. This work was supported in part by the US National Institutes of Health Director’s Pioneer award (to S.R.Q.) and a Fulbright award (to D.G.).

Funding

We thank members of the Stanford microfluidics foundry for help with device fabrication. This work was supported in part by the US National Institutes of Health Director’s Pioneer award (to S.R.Q.) and a Fulbright award (to D.G.).

Funders	Funder number
National Institutes of Health
National Human Genome Research Institute	R01HG002644
NIH Office of the Director	DP1OD000251

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10.1038/nmeth.1289

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An in vitro microfluidic approach to generating protein-interaction networks

Abstract

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