An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Yulia Shenberger; Lada Gevorkyan-Airapetov; Melanie Hirsch; Lukas Hofmann; Sharon Ruthstein

doi:10.1039/d3cc03047d

An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Yulia Shenberger
, Lada Gevorkyan-Airapetov
, Melanie Hirsch
, Lukas Hofmann
, Sharon Ruthstein

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.

Original language	English
Pages (from-to)	10524-10527
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	70
DOIs	https://doi.org/10.1039/d3cc03047d
State	Published - 29 Aug 2023

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Publisher Copyright:
© 2023 The Royal Society of Chemistry.

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10.1039/d3cc03047d

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abstract = "EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.",

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AU - Hirsch, Melanie

AU - Hofmann, Lukas

AU - Ruthstein, Sharon

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AB - EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.

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An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Abstract

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