An immunoenzyme quantitative assay for the antiviral effect of interferons

Jacob Shoham, Miriam Cohen, David Wallach

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


A technique is described for measurement of the antiviral activity of interferon by an immunoenzymatic assay for viral proteins. Cells treated by tested samples of interferon (IFN) are infected with vesicular stomatitis virus (VSV) and following the development of viral cytopathy are lysed by the addition of deoxycholate and then transferred into ELISA microplates. The viral proteins bind effectively to the microplates proportionally to their level in the culture and may be measured by incubating the plates sequentially with (1) rabbit antiserum against VSV, (2) a conjugate of alkaline phosphatase either to protein A or to an antibody against rabbit IgG and (3) p-nitrophenylphosphate. This procedure may be further simplified by using antibodies against VSV to which alkaline phosphatase has been directly conjugated. We found this immunoenzyme assay to be superior to the 'cytophatic effect inhibition' assay in precision and sensitivity and in being independent of the effectiveness of viral cytopathy.

Original languageEnglish
Pages (from-to)279-287
Number of pages9
JournalJournal of Immunological Methods
Issue number1
StatePublished - 3 Aug 1984
Externally publishedYes


  • interferon assay
  • viral protein assay


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