An expedient synthesis of CMF-019: (s)-5-methyl-3-{1-(pentan-3-yl)-2-(thiophen-2-ylmethyl)-1h-benzo[d]imidazole-5-carboxamido}hexanoic acid, a potent apelin receptor (APJ) agonist

Lena Trifonov, Michal Afri, Krzysztof Palczewski, Edward E. Korshin, Arie Gruzman

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Background: Apelin receptor (APJ) is a G protein-coupled receptor (GPCR) activated by the endogenous peptide apelin. The apelin–APJ system has emerged as an important regulator of cardiovascular homeostasis. Recently, a potent benzimidazole-derived apelin peptidomimetic, CMF-019, was patented but without a comprehensive description of its synthesis and a complete spectroscopic characterization of the intermediates. Objective: Here, a detailed preparation of CMF-019 through a modified and improved synthetic pathway is described. Method: In particular, the benzimidazole ring in 7 was tailored by the condensation of methyl 3-amino-4-(pentan-3-ylamino)benzoate (4) with (thiophene-2-yl)acetimidate salt 6. Saponification of 7 and the subsequent condensation of the free acid 8 with the corresponding enantiopure β-amino acid methyl ester generated methyl (S)-5-methyl-3-{1-(pentan-3-yl)-2-(thiophen-2-ylmethyl)-1H-benzo[d]imidazole-5-carboxamido}hexanoate (9). Hydrolysis of the latter with KOH in THF/water, followed by HPLC-purification, afforded the desired product, CMF-019 (potassium salt) 10. Results & Conclusion: The approach reported herein enables preparation of 10 at a total yield of 12% over seven linear steps. Additionally, it does not require applying expensive designated microwave reactors and high-pressure hydrogenators. Thus, the elaborate synthesis provides a latent availability of potent agonist 10 for further exploring the physiologically essential apelin-APJ system.

Original languageEnglish
Pages (from-to)688-694
Number of pages7
JournalMedicinal Chemistry
Volume14
Issue number7
DOIs
StatePublished - 2018

Bibliographical note

Funding Information:
We thank Gila Levy for her assistance with the polarimetric measurements and Steve Manch for English editing. This study was supported by a Bar-Ilan University new faculty grant (AG), by the Israel Ministry of Immigration and Integration through Kamea fellowship (EEK), and by EY024864 and EY027283 grants (KP).

Publisher Copyright:
© 2018 Bentham Science Publishers.

Keywords

  • Apelin
  • Apelin receptor
  • Benzimidazole scaffold
  • CMF-019
  • Expedient synthesis
  • Peptidomimetics

Fingerprint

Dive into the research topics of 'An expedient synthesis of CMF-019: (s)-5-methyl-3-{1-(pentan-3-yl)-2-(thiophen-2-ylmethyl)-1h-benzo[d]imidazole-5-carboxamido}hexanoic acid, a potent apelin receptor (APJ) agonist'. Together they form a unique fingerprint.

Cite this