Abstract
A simple procedure for the preparation and purification of trans-[14C]geranylgeranyl pyrophosphate from dl-[14C]mevalonate is described. This procedure involves biosynthesis of trans-[14C]geranylgeraniol from dl-[14C]mevalonate by cell-free extracts of the fungus Gibberella fujikuroi and chemical synthesis of trans-[14C]geranylgeranyl pyrophosphate from trans-[14C]geranylgeraniol. The purification of trans-[14C]geranylgeraniol is achieved by chromatography on a silver nitrate-impregnated silica gel column. The pyrophosphate ester is purified on a silica gel column.
| Original language | English |
|---|---|
| Pages (from-to) | 248-252 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 92 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Jan 1979 |
| Externally published | Yes |