An efficient method for the synthesis and purification of trans-[14C]geranylgeranyl pyrophosphate

Yedidya Gafni, Ishaiahu Shechter

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

A simple procedure for the preparation and purification of trans-[14C]geranylgeranyl pyrophosphate from dl-[14C]mevalonate is described. This procedure involves biosynthesis of trans-[14C]geranylgeraniol from dl-[14C]mevalonate by cell-free extracts of the fungus Gibberella fujikuroi and chemical synthesis of trans-[14C]geranylgeranyl pyrophosphate from trans-[14C]geranylgeraniol. The purification of trans-[14C]geranylgeraniol is achieved by chromatography on a silver nitrate-impregnated silica gel column. The pyrophosphate ester is purified on a silica gel column.

Original languageEnglish
Pages (from-to)248-252
Number of pages5
JournalAnalytical Biochemistry
Volume92
Issue number1
DOIs
StatePublished - 1 Jan 1979
Externally publishedYes

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