Amplified electronic transduction of oligonucleotide interactions: Novel routes for Tay-Sachs biosensors

Amos Bardea, Arie Dagan, Itamar Willner

Research output: Contribution to journalArticlepeer-review

40 Scopus citations

Abstract

Two different configurations for the amplified microgravimetric analysis of oligonucleotides (DNA) are presented. A thiol-tagged oligonucleotide, (1), is assembled on a Au-quartz electrode, and the complementary oligonucleotide (2) is microgravimetrically sensed by the crystal. Formation of the ds-oligonucleotide assembly is amplified using mouse anti-dsDNA antibody and goat anti-mouse Fc antibody. Alternatively, a biotin-labeled oligonucleotide, (4), is used to form the ds-oligonucleotide. Biotin is used to amplify the formation of the ds-complex. An electrochemical method, using cytochrome c as redox probe, to assay the formation of the ds-oligonucleotide assembly, is described. The 5'-carboxylic acid-functionalized oligonucleotide (5) is coupled to a cystamine monolayer associated with an Au-electrode. The electrostatic attraction of cytochrome c to the ds-oligonucleotide complex facilitates the interfacial electron transfer to cytochrome c. Formation of the ds-oligonucleotide assembly is detected electrochemically using differential pulse voltammetry. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)33-43
Number of pages11
JournalAnalytica Chimica Acta
Volume385
Issue number1-3
DOIs
StatePublished - 5 Apr 1999
Externally publishedYes

Bibliographical note

Funding Information:
This study was supported by The Robert Szold Foundation, The Hebrew University of Jerusalem, Israel.

Keywords

  • Anti-dsDNA antibody
  • Biotin-labeled oligonucleotide
  • DNA-sensor
  • Electrochemical DNA-sensor
  • Oligonucleotide monolayer
  • Quartz-crystal-microbalance
  • Tay-Sachs

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