Amplified analysis of DNA by the autonomous assembly of polymers consisting of DNAzyme wires

Fuan Wang; Johann Elbaz; Ron Orbach; Nimrod Magen; Itamar Willner

doi:10.1021/ja2076789

Amplified analysis of DNA by the autonomous assembly of polymers consisting of DNAzyme wires

Fuan Wang, Johann Elbaz, Ron Orbach, Nimrod Magen, Itamar Willner

Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

312 Scopus citations

Abstract

A systematic study of the amplified optical detection of DNA by Mg ²⁺-dependent DNAzyme subunits is described. The use of two DNAzyme subunits and the respective fluorophore/quencher-modified substrate allows the detection of the target DNA with a sensitivity corresponding to 1 × 10 ^-9 M. The use of two functional hairpin structures that include the DNAzyme subunits in a caged, inactive configuration leads, in the presence of the target DNA, to the opening of one of the hairpins and to the activation of an autonomous cross-opening process of the two hairpins, which affords polymer DNA wires consisting of the Mg ²⁺-dependent DNAzyme subunits. This amplification paradigm leads to the analysis of the target DNA with a sensitivity corresponding to 1 × 10 ^-14 M. The amplification mixture composed of the two hairpins can be implemented as a versatile sensing platform for analyzing any gene in the presence of the appropriate hairpin probe. This is exemplified with the detection of the BRCA1 oncogene.

Original language	English
Pages (from-to)	17149-17151
Number of pages	3
Journal	Journal of the American Chemical Society
Volume	133
Issue number	43
DOIs	https://doi.org/10.1021/ja2076789
State	Published - 2 Nov 2011
Externally published	Yes

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abstract = "A systematic study of the amplified optical detection of DNA by Mg 2+-dependent DNAzyme subunits is described. The use of two DNAzyme subunits and the respective fluorophore/quencher-modified substrate allows the detection of the target DNA with a sensitivity corresponding to 1 × 10 -9 M. The use of two functional hairpin structures that include the DNAzyme subunits in a caged, inactive configuration leads, in the presence of the target DNA, to the opening of one of the hairpins and to the activation of an autonomous cross-opening process of the two hairpins, which affords polymer DNA wires consisting of the Mg 2+-dependent DNAzyme subunits. This amplification paradigm leads to the analysis of the target DNA with a sensitivity corresponding to 1 × 10 -14 M. The amplification mixture composed of the two hairpins can be implemented as a versatile sensing platform for analyzing any gene in the presence of the appropriate hairpin probe. This is exemplified with the detection of the BRCA1 oncogene.",

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AU - Elbaz, Johann

AU - Orbach, Ron

AU - Magen, Nimrod

AU - Willner, Itamar

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AB - A systematic study of the amplified optical detection of DNA by Mg 2+-dependent DNAzyme subunits is described. The use of two DNAzyme subunits and the respective fluorophore/quencher-modified substrate allows the detection of the target DNA with a sensitivity corresponding to 1 × 10 -9 M. The use of two functional hairpin structures that include the DNAzyme subunits in a caged, inactive configuration leads, in the presence of the target DNA, to the opening of one of the hairpins and to the activation of an autonomous cross-opening process of the two hairpins, which affords polymer DNA wires consisting of the Mg 2+-dependent DNAzyme subunits. This amplification paradigm leads to the analysis of the target DNA with a sensitivity corresponding to 1 × 10 -14 M. The amplification mixture composed of the two hairpins can be implemented as a versatile sensing platform for analyzing any gene in the presence of the appropriate hairpin probe. This is exemplified with the detection of the BRCA1 oncogene.

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Amplified analysis of DNA by the autonomous assembly of polymers consisting of DNAzyme wires

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