TY - JOUR
T1 - Alternating laser excitation for solution-based single-molecule FRET
AU - Kapanidis, Achillefs
AU - Majumdar, Devdoot
AU - Heilemann, Mike
AU - Nir, Eyal
AU - Weiss, Shimon
N1 - Publisher Copyright:
© 2015 Cold Spring Harbor Laboratory Press.
PY - 2015/11/2
Y1 - 2015/11/2
N2 - Single-molecule fluorescence resonance energy transfer (smFRET) has been widely applied to the study of fluorescently labeled biomolecules on surfaces and in solution. Sorting single molecules based on fluorescent dye stoichiometry provides one with further layers of information and also enables “filtering” of unwanted molecules from the analysis. We accomplish this sorting by using alternating laser excitation (ALEX) in combination with smFRET measurements; here we describe the implementation of these methodologies for the study of biomolecules in solution.
AB - Single-molecule fluorescence resonance energy transfer (smFRET) has been widely applied to the study of fluorescently labeled biomolecules on surfaces and in solution. Sorting single molecules based on fluorescent dye stoichiometry provides one with further layers of information and also enables “filtering” of unwanted molecules from the analysis. We accomplish this sorting by using alternating laser excitation (ALEX) in combination with smFRET measurements; here we describe the implementation of these methodologies for the study of biomolecules in solution.
UR - http://www.scopus.com/inward/record.url?scp=84946017831&partnerID=8YFLogxK
U2 - 10.1101/pdb.top086405
DO - 10.1101/pdb.top086405
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C2 - 26527772
AN - SCOPUS:84946017831
SN - 1940-3402
VL - 2015
SP - 979
EP - 987
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 11
ER -