Alteration of mitochondrial membrane potential in photosensitized cardiocytes monitored by fast-response fluorescent probe

Mariusz Adamek, Asher Shainberg, Fidi Schwartz, Shimon Rochkind

Research output: Contribution to journalConference articlepeer-review

Abstract

Mitochondria constitute well-established target for photodynamic action provided that duration of incubation time with hydrophobic photosensitizer is long enough (18-24 hrs) to enable incorporation of the dye into these organelles. It was found that 1 hour incubation of rat cardiocytes with haematoporphyrin dihydrochloride (Hp) followed by low-energy HeNe laser irradiation (ED: 0.3, 1.0 or 3.0 J/cm2), affected mitochondrial membrane potential. The latter was monitored by means of the fast-response fluorescent probe DASPMI and the dynamic video imaging system equipped with frame-grabbing and analyzing software. The time-course of mitochondrial membrane potential decrease exhibited dependence on both: Hp concentration and ED used. In cells incubated with 1 μg/ml Hp a decrease of DASPMI fluorescence occurred during 8-15 mins and was preceded by oscillations of fluorescence intensity. For cells incubated with 100 μg/ml Hp disappearance of discrete pattern of mitochondrial fluorescence was observed 1-2 mins after irradiation.

Original languageEnglish
Pages (from-to)146-154
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3191
DOIs
StatePublished - 1997
EventPhotochemotherapy: Photodynamic Therapy and Other Modalities III - San Remo, Italy
Duration: 4 Sep 19974 Sep 1997

Keywords

  • Cardiocytes
  • Fluorescent probe
  • Haematoporphyrin
  • Mitochondrial membrane potential
  • Photosensitization

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