TY - JOUR
T1 - Aligning the μs-ALEX setup
AU - Kapanidis, Achillefs
AU - Majumdar, Devdoot
AU - Heilemann, Mike
AU - Nir, Eyal
AU - Weiss, Shimon
N1 - Publisher Copyright:
© 2015 Cold Spring Harbor Laboratory Press.
PY - 2015/11/2
Y1 - 2015/11/2
N2 - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.
AB - To achieve single-molecule sensitivity and thus have the ability to detect single diffusing fluorophores, careful alignment of the microsecond-alternating laser excitation (μs-Alex) setup is crucial. The following protocol describes routine alignment for 2c-ALEX (532 nm/635 nm) with spectral windows G550–620R650–750.
UR - http://www.scopus.com/inward/record.url?scp=84946040192&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot086421
DO - 10.1101/pdb.prot086421
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C2 - 26527766
AN - SCOPUS:84946040192
SN - 1940-3402
VL - 2015
SP - 1027
EP - 1028
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 11
ER -