TY - JOUR
T1 - AKT1 E17 K pleckstrin homology domain mutation in urothelial carcinoma
AU - Zilberman, Dorit E.
AU - Cohen, Yoram
AU - Amariglio, Ninette
AU - Fridman, Edward
AU - Ramon, Jacob
AU - Rechavi, Gideon
PY - 2009/5
Y1 - 2009/5
N2 - The PI3 K/AKT pathway is frequently activated in human cancer. Recently, a G to A point mutation (E17 K) was found in the pleckstrin homology domain of AKT1. We aimed to explore this mutation in cases of urothelial carcinoma. Using chip-based matrix-assisted laser desorption-time-of-flight (MALDI-TOF) mass spectrometer, AKT1 E17 K mutation was searched in 26 total RNA samples obtained from 26 patients known to have urothelial carcinoma. Mutation was found in one out of 26 (3.8%) patients - a 46 year old female with a low grade transitional cell carcinoma located to the lamina propria (Ta disease). Our finding is in line with previous studies showing AKT1 E17 K mutation to be rare. Yet, further studies are required to determine whether this mutation is indeed related to less aggressive disease and carries better prognosis.
AB - The PI3 K/AKT pathway is frequently activated in human cancer. Recently, a G to A point mutation (E17 K) was found in the pleckstrin homology domain of AKT1. We aimed to explore this mutation in cases of urothelial carcinoma. Using chip-based matrix-assisted laser desorption-time-of-flight (MALDI-TOF) mass spectrometer, AKT1 E17 K mutation was searched in 26 total RNA samples obtained from 26 patients known to have urothelial carcinoma. Mutation was found in one out of 26 (3.8%) patients - a 46 year old female with a low grade transitional cell carcinoma located to the lamina propria (Ta disease). Our finding is in line with previous studies showing AKT1 E17 K mutation to be rare. Yet, further studies are required to determine whether this mutation is indeed related to less aggressive disease and carries better prognosis.
UR - http://www.scopus.com/inward/record.url?scp=64849084242&partnerID=8YFLogxK
U2 - 10.1016/j.cancergencyto.2009.01.009
DO - 10.1016/j.cancergencyto.2009.01.009
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C2 - 19389506
AN - SCOPUS:64849084242
SN - 0165-4608
VL - 191
SP - 34
EP - 37
JO - Cancer Genetics and Cytogenetics
JF - Cancer Genetics and Cytogenetics
IS - 1
ER -
