Advances in the development of a novel method to be used in proteomics using gold nanobeads

Heidelinde R.C. Dietrich; Ian T. Young; Yuval Garini

doi:10.1117/12.645908

Advances in the development of a novel method to be used in proteomics using gold nanobeads

Heidelinde R.C. Dietrich
, Ian T. Young
, Yuval Garini

Delft University of Technology

Research output: Contribution to journal › Conference article › peer-review

1 Scopus citations

Abstract

The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact that there are only a few techniques that permit the study of these interactions. In this report we describe the development of a research tool based on tethered bead motion and Resonance Light Scattering (RLS) from gold beads. This method permits the study of DNA-protein interactions and the screening of proteins binding to a specific DNA sequence.

Original language	English
Article number	60920C
Journal	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6092
DOIs	https://doi.org/10.1117/12.645908
State	Published - 2006
Externally published	Yes
Event	Ultrasensitive and Single-Molecule Detection Technologies - San Jose, CA, United States Duration: 21 Jan 2006 → 24 Jan 2006

Keywords

Molecular interactions
Quantitative microscopy
Resonance light scattering
Single molecule detection

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10.1117/12.645908

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title = "Advances in the development of a novel method to be used in proteomics using gold nanobeads",

abstract = "The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact that there are only a few techniques that permit the study of these interactions. In this report we describe the development of a research tool based on tethered bead motion and Resonance Light Scattering (RLS) from gold beads. This method permits the study of DNA-protein interactions and the screening of proteins binding to a specific DNA sequence.",

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AU - Dietrich, Heidelinde R.C.

AU - Young, Ian T.

AU - Garini, Yuval

PY - 2006

Y1 - 2006

N2 - The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact that there are only a few techniques that permit the study of these interactions. In this report we describe the development of a research tool based on tethered bead motion and Resonance Light Scattering (RLS) from gold beads. This method permits the study of DNA-protein interactions and the screening of proteins binding to a specific DNA sequence.

AB - The study of DNA-protein interactions is gaining increased attention due to their importance in cellular processes. Only a well-functioning interaction guaranties that such a process can take place without errors. So far, only a small percentage of these interactions have been unraveled, partially due to their complexity but also due to the fact that there are only a few techniques that permit the study of these interactions. In this report we describe the development of a research tool based on tethered bead motion and Resonance Light Scattering (RLS) from gold beads. This method permits the study of DNA-protein interactions and the screening of proteins binding to a specific DNA sequence.

KW - Molecular interactions

KW - Quantitative microscopy

KW - Resonance light scattering

KW - Single molecule detection

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T2 - Ultrasensitive and Single-Molecule Detection Technologies

Y2 - 21 January 2006 through 24 January 2006

ER -

Advances in the development of a novel method to be used in proteomics using gold nanobeads

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Keywords

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