Activity-dependent A-to-I RNA editing in rat cortical neurons

Neville E. Sanjana, Erez Y. Levanon, Emily A. Hueske, Jessica M. Ambrose, Jin Billy Li

Research output: Contribution to journalComment/debate

36 Scopus citations


Changes in neural activity influence synaptic plasticity/scaling, gene expression, and epigenetic modifications. We present the first evidence that short-term and persistent changes in neural activity can alter adenosine-to-inosine (A-to-I) RNA editing, a posttranscriptional site-specific modification found in several neuron-specific transcripts. In rat cortical neuron cultures, activity-dependent changes in A-to-I RNA editing in coding exons are present after 6 hr of high potassium depolarization but not after 1 hr and require calcium entry into neurons. When treatments are extended from hours to days, we observe a negative feedback phenomenon: Chronic depolarization increases editing at many sites and chronic silencing decreases editing. We present several different modulations of neural activity that change the expression of different mRNA isoforms through editing.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
Issue number1
StatePublished - 1 Sep 2012


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