Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

Thomas Dertinger; Ryan Colyer; Robert Vogel; Jörg Enderlein; Shimon Weiss

doi:10.1364/OE.18.018875

Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Jörg Enderlein, Shimon Weiss

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of nearinfrared quantum dot labeled tubulin-network of 3T3 fibroblasts.

Original language	English
Pages (from-to)	18875-18885
Number of pages	11
Journal	Optics Express
Volume	18
Issue number	18
DOIs	https://doi.org/10.1364/OE.18.018875
State	Published - 30 Aug 2010
Externally published	Yes

Funding

Funders	Funder number
National Institute of General Medical Sciences	R01GM086197
National Institute of Biomedical Imaging and Bioengineering	R01EB000312

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10.1364/OE.18.018875

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Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

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