Achieving increased resolution and more pixels with Superresolution Optical Fluctuation Imaging (SOFI)

Thomas Dertinger, Ryan Colyer, Robert Vogel, Jörg Enderlein, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

170 Scopus citations

Abstract

Superresolution Optical Fluctuation Imaging (SOFI) as initially demonstrated allows for a resolution enhancement in imaging by a factor of square-root of two. Here, we demonstrate how to increase the resolution of SOFI images by re-weighting the Optical Transfer Function (OTF). Furthermore, we demonstrate how cross-cumulants can be exploited to obtain a fair approximation of the underlying Point-Spread Function. We show a two-fold increase of resolution (over the diffraction limit) of nearinfrared quantum dot labeled tubulin-network of 3T3 fibroblasts.

Original languageEnglish
Pages (from-to)18875-18885
Number of pages11
JournalOptics Express
Volume18
Issue number18
DOIs
StatePublished - 30 Aug 2010
Externally publishedYes

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