Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy

K. Shanmugha Rajan; Ganesan Velmurugan; Pandi Gopal; Tharmarajan Ramprasath; D. D.Venkatesh Babu; S. Krithika; Y. Christy Jenifer; Allen Freddy; George William; Krishnan Kalpana; Subbiah Ramasamy

doi:10.1016/j.hlc.2016.02.015

Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy

K. Shanmugha Rajan, Ganesan Velmurugan, Pandi Gopal, Tharmarajan Ramprasath, D. D.Venkatesh Babu, S. Krithika, Y. Christy Jenifer, Allen Freddy, George William, Krishnan Kalpana, Subbiah Ramasamy

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57 Scopus citations

Abstract

Background The discovery of PIWI-interacting RNAs (piRNAs) has fundamentally changed our understanding of post transcriptional regulation of transposons and other genes. Unlike miRNA and siRNA, the piRNAs are the most abundant but least studied RNA species in mammals. Although the expression of PIWI proteins and piRNAs has long been regarded as germline specific, increasing evidences suggest the expression of piRNAs in somatic cells. Methods In this study, the small RNA sequencing executed during induction of cardiac hypertrophy in both in vivo and in vitro conditions were annotated for the expression of piRNAs. The expression of piRNAs was validated by qPCR and RNA immunoprecipitation. In addition, the presence of piRNAs in circulation of myocardial infarction patients was studied by qPCR. Results We identified an abundant and altered expression of piRNAs during cardiac hypertrophy. The differentially expressed piRNAs was validated by qPCR and RNA immunoprecipitation. The significantly and differentially expressed piRNAs were predicted to target different retrotransposons and mRNAs in the rat genome. The detection of specific piRNA in serum of myocardial infarction patients suggests the potential of piRNA for diagnosis. Conclusion Overall this study is the first to provide a whole-genome analysis of the large repertoire of piRNAs in the cardiac system and this would pave a new path to understanding the molecular aetiology of piRNA and retrotransposons in the physiology and pathology of the cardiac system.

Original language	English
Pages (from-to)	1013-1020
Number of pages	8
Journal	Heart Lung and Circulation
Volume	25
Issue number	10
DOIs	https://doi.org/10.1016/j.hlc.2016.02.015
State	Published - 1 Oct 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ)

Funding

The authors duly thank DBT (BT/PR 15077/GBD/27/300/2011) and SERB (SR/FT/LS-48/2011, SR/FT/LS-37/2011) Govt. of India for their funding and DBT-IPLS, UGC-NRCBS, -CEGS, -CAS, -UPE, and DST-PURSE for funding and instrumentation facility. We acknowledge Mr. Adam Brown, a native English speaker for correcting the manuscript.

Funders	Funder number
DBT-IPLS
DST-PURSE
UGC-NRCBS
Chinese Academy of Sciences

Keywords

Cardiac hypertrophy
Retrotransposons
Small RNA deep sequencing
Small non-coding RNA
piRNA

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10.1016/j.hlc.2016.02.015

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title = "Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy",

abstract = "Background The discovery of PIWI-interacting RNAs (piRNAs) has fundamentally changed our understanding of post transcriptional regulation of transposons and other genes. Unlike miRNA and siRNA, the piRNAs are the most abundant but least studied RNA species in mammals. Although the expression of PIWI proteins and piRNAs has long been regarded as germline specific, increasing evidences suggest the expression of piRNAs in somatic cells. Methods In this study, the small RNA sequencing executed during induction of cardiac hypertrophy in both in vivo and in vitro conditions were annotated for the expression of piRNAs. The expression of piRNAs was validated by qPCR and RNA immunoprecipitation. In addition, the presence of piRNAs in circulation of myocardial infarction patients was studied by qPCR. Results We identified an abundant and altered expression of piRNAs during cardiac hypertrophy. The differentially expressed piRNAs was validated by qPCR and RNA immunoprecipitation. The significantly and differentially expressed piRNAs were predicted to target different retrotransposons and mRNAs in the rat genome. The detection of specific piRNA in serum of myocardial infarction patients suggests the potential of piRNA for diagnosis. Conclusion Overall this study is the first to provide a whole-genome analysis of the large repertoire of piRNAs in the cardiac system and this would pave a new path to understanding the molecular aetiology of piRNA and retrotransposons in the physiology and pathology of the cardiac system.",

keywords = "Cardiac hypertrophy, Retrotransposons, Small RNA deep sequencing, Small non-coding RNA, piRNA",

author = "Rajan, {K. Shanmugha} and Ganesan Velmurugan and Pandi Gopal and Tharmarajan Ramprasath and Babu, {D. D.Venkatesh} and S. Krithika and Jenifer, {Y. Christy} and Allen Freddy and George William and Krishnan Kalpana and Subbiah Ramasamy",

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AU - Velmurugan, Ganesan

AU - Gopal, Pandi

AU - Ramprasath, Tharmarajan

AU - Babu, D. D.Venkatesh

AU - Krithika, S.

AU - Jenifer, Y. Christy

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AU - William, George

AU - Kalpana, Krishnan

AU - Ramasamy, Subbiah

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Abundant and Altered Expression of PIWI-Interacting RNAs during Cardiac Hypertrophy

Abstract

Bibliographical note

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Keywords

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