A-to-I RNA editing in the earliest-diverging eumetazoan phyla

Hagit T. Porath; Amos A. Schaffer; Paulina Kaniewska; Shahar Alon; Eli Eisenberg; Joshua Rosenthal; Erez Y. Levanon; Oren Levy

doi:10.1093/molbev/msx125

A-to-I RNA editing in the earliest-diverging eumetazoan phyla

Hagit T. Porath, Amos A. Schaffer, Paulina Kaniewska, Shahar Alon, Eli Eisenberg, Joshua Rosenthal, Erez Y. Levanon, Oren Levy

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

The highly conservedADARenzymes, found in allmulticellularmetazoans, catalyze the editing ofmRNAtranscripts by the deamination of adenosines to inosines. This type of editing has two general outcomes: site specific editing,which frequently leads to recoding, and clustered editing, which is usually found in transcribed genomic repeats. Here, for the first time, we looked for both editing of isolated sites and clustered, non-specific sites in a basalmetazoan, the coral Acropora millepora during spawning event, in order to reveal its editing pattern.We found that the coral editome resembles the mammalian one: it contains more than 500,000 sites, virtually all of which are clustered in non-coding regions that are enriched for predicted dsRNA structures. RNA editing levels were increased during spawning and increased further still in newly released gametes. This may suggest that editing plays a role in introducing variability in coral gametes.

Original language	English
Pages (from-to)	1890-1901
Number of pages	12
Journal	Molecular Biology and Evolution
Volume	34
Issue number	8
Early online date	8 Apr 2017
DOIs	https://doi.org/10.1093/molbev/msx125
State	Published - 1 Aug 2017

Bibliographical note

Publisher Copyright:
© The Author 2017.

Funding

We thank Heron Island Research Station staff for their help during our spawning experiments. This work was supported by the Australian Research Council (to PK), the European Research Council (grant 311257), the I-CORE Program of the Planning and Budgeting Committee in Israel (grants 41/ 11 and 1796/12), and the Israel Science Foundation (1380/14). The sequencing data of the one sample coral was deposited in the Sequence Read Archive (SRA), under accession number SRP080785.

Funders	Funder number
Sugar Research Australia	SRP080785
Seventh Framework Programme	311257
European Commission
Australian Research Council
Israel Science Foundation	1380/14
Israel Ports Company	1796/12, 41/ 11
Israeli Centers for Research Excellence

Keywords

ADAR
Coral
Evolution
RNA editing

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10.1093/molbev/msx125

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abstract = "The highly conservedADARenzymes, found in allmulticellularmetazoans, catalyze the editing ofmRNAtranscripts by the deamination of adenosines to inosines. This type of editing has two general outcomes: site specific editing,which frequently leads to recoding, and clustered editing, which is usually found in transcribed genomic repeats. Here, for the first time, we looked for both editing of isolated sites and clustered, non-specific sites in a basalmetazoan, the coral Acropora millepora during spawning event, in order to reveal its editing pattern.We found that the coral editome resembles the mammalian one: it contains more than 500,000 sites, virtually all of which are clustered in non-coding regions that are enriched for predicted dsRNA structures. RNA editing levels were increased during spawning and increased further still in newly released gametes. This may suggest that editing plays a role in introducing variability in coral gametes.",

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AU - Eisenberg, Eli

AU - Rosenthal, Joshua

AU - Levanon, Erez Y.

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A-to-I RNA editing in the earliest-diverging eumetazoan phyla

Abstract

Bibliographical note

Funding

Keywords

Access to Document

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