A TILLING population for cucumber forward and reverse genetics

R Fraenkel, I. Kovalski, C. Troadec, A. Bendahmane, R. Perl-Treves, N. Sari (Editor), I. Solmaz (Editor), V. Aras (Editor)

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review


Ordered collections of mutants serve as invaluable tools in biology, and in functional genomics in particular. TILLING (targeting induced local lesions in genomes) provides an efficient method to study the possible phenotype controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. We report the development of a TILLING cucumber population, prepared by EMS mutagenesis in the Poinsett-76 genetic background. The population was evaluated by screening morphological mutations, and its suitability for detecting single nucleotide polymorphism in selected genes has been tested. Such previously-unavailable asset in cucumber can be exploited for diverse breeding and research purposes
Original languageEnglish
Title of host publicationCucurbitaceae 2012. Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae
PublisherCukurova University, Faculty of Medicine
ISBN (Print)9786056329708
StatePublished - 2012

Bibliographical note

Place of conference:Turkey


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