A system is described for the precise continuous measurement of oxygen consumption and photo-synthetic oxygen evolution in a well defined light field with a polarographic oxygen electrode. The procedure uses a parallel light beam to irradiate samples, a relatively short pathlength and temperature regulation. Maximal sensitivity of the O2-mcasurement is 1.4 μmol O2/mV at 20°C. The O2-chamber and the O2-amplifier are presented in full detail together with a typical recorder tracing of a photosynthesis-irradiance curve.
Bibliographical noteFunding Information:
The system we present here was originally developed at Brookhaven National Laboratory and is the 'final' outcome of a long evolution of both O2-chamber and O2-amplifer as well as their 'symbiosis' to which many people have contributed. The mention of product names does not imply endorsement. Special thanks are due to Dr Rudolf Slovacek for advice on the design of the operational amplifier. This research was performed under the auspices of the United States Department of Energy under Contract No. DE-AC02-76CH00016.