A simple way to measure alterations in reward-seeking behavior using Drosophila melanogaster

Shir Zer, Julia Ryvkin, Harel J. Wilner, Hila Zak, Anat Shmueli, Galit Shohat-Ophir

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We describe a protocol for measuring ethanol self-administration in fruit flies (Drosophila melanogaster) as a proxy for changes in reward states. We demonstrate a simple way to tap into the fly reward system, modify experiences related to natural reward, and use voluntary ethanol consumption as a measure for changes in reward states. The approach serves as a relevant tool to study the neurons and genes that play a role in experience-mediated changes of internal state. The protocol is composed of two discrete parts: exposing the flies to rewarding and nonrewarding experiences, and assaying voluntary ethanol consumption as a measure of the motivation to obtain a drug reward. The two parts can be used independently to induce the modulation of experience as an initial step for further downstream assays or as an independent two-choice feeding assay, respectively. The protocol does not require a complicated setup and can therefore be applied in any laboratory with basic fly culture tools.

Original languageEnglish
Article numbere54910
JournalJournal of Visualized Experiments
Volume2016
Issue number118
DOIs
StatePublished - 15 Dec 2016

Bibliographical note

Publisher Copyright:
© 2016 Journal of Visualized Experiments.

Keywords

  • Addiction
  • Alcohol
  • Behavior
  • Consumption
  • Courtship
  • Courtship suppression
  • Drosophila
  • Experience
  • Issue 118
  • Mating
  • Neuroscience
  • Preference
  • Social

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