A role for G-proteins in the epidermal growth factor stimulation of phospholipase A2 in rat kidney mesangial cells

Nashrudeen Hack, Paula Clayman, Karl Skorecki

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We have previously demonstrated phospholipase C (PLC) independent activation of phospholipase A2(PLA2) by epidermal growth factor (EGF) in glomerular mesangial cells in culture. In the current study using glass beads to permeabilize [3H]- or [14C]-arachidonate labelled mesangial cells we demonstrate that guanine nucleotides modulate the EGF-mediated stimulation of arachidonic acid release (75% inhibition with 100 μM GDPβS and 108% augmentation with 100 μM GTPγS). GTPγS alone stimulated both the release of free arachidonic acid and production of diacylglycerol (DAG), while EGF itself neither stimulated DAG nor augmented the DAG response to GTPγS. These findings suggest the intermediacy of a G-protein in PLC-independent stimulation of PLA2 by a growth factor, and provide a model system for determining the relationship between G-protein intermediacy and the intrinsic tyrosine kinase activity of the growth factor receptor.

Original languageEnglish
Pages (from-to)353-362
Number of pages10
JournalBioscience Reports
Volume10
Issue number4
DOIs
StatePublished - Aug 1990
Externally publishedYes

Keywords

  • G-proteins
  • epidermal growth factor
  • glass beads
  • mesangial cells
  • phospholipase A

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