Land plants evolved to quickly sense and adapt to temperature changes, such as hot days and cold nights. Given that calcium (Ca2+) signaling networks are implicated in most abiotic stress responses, heat-triggered changes in cytosolic Ca2+ were investigated in Arabidopsis leaves and pollen. Plants were engineered with a reporter called CGf, a ratiometric, genetically encoded Ca2+ reporter with an mCherry reference domain fused to an intensiometric Ca2+ reporter GCaMP6f. Relative changes in [Ca2+]cyt were estimated based on CGf’s apparent KD around 220 nM. The ratiometric output provided an opportunity to compare Ca2+ dynamics between different tissues, cell types, or subcellular locations. In leaves, CGf detected heat-triggered cytosolic Ca2+ signals, comprised of three different signatures showing similarly rapid rates of Ca2+ influx followed by differing rates of efflux (50% durations ranging from 5 to 19 min). These heat-triggered Ca2+ signals were approximately 1.5-fold greater in magnitude than blue light-triggered signals in the same leaves. In contrast, growing pollen tubes showed two different heat-triggered responses. Exposure to heat caused tip-focused steady growth [Ca2+]cyt oscillations to shift to a pattern characteristic of a growth arrest (22%), or an almost undetectable [Ca2+]cyt (78%). Together, these contrasting examples of heat-triggered Ca2+ responses in leaves and pollen highlight the diversity of Ca2+ signals in plants, inviting speculations about their differing kinetic features and biological functions.
|Journal||Frontiers in Plant Science|
|State||Published - 17 Dec 2021|
Bibliographical noteFunding Information:
This work was supported by the United States Department of Agriculture (HATCH grant no. NEV00384 to JH), the National Science Foundation (IOS grant no. 1656774 to JH, and MCB grant no. 2016143 to W-GC), and US-Israel Binational Agricultural Research and Development Fund (BARD grant no. IS-4652-13 to JH and GM).
Copyright © 2021 Weigand, Kim, Brown, Medina, Mares, Miller, Harper and Choi.
- heat stress
- mCherry fused GCaMP6f
- pollen tube imaging
- ratiometric calcium reporter CGf
- single cell imaging
- whole rosette imaging